摘要
本研究探讨在小白菜栽培时,分别添加果蔬加工废弃物生产的堆肥和化肥对土壤微生物区系的影响。利用扩增16SrRNA基因来比较微生物群落的技术,分离出所有群落DNA,再以此DNA为模板,以专一性引物扩增出16SrRNA基因(rDNA)。随后建立16SrDNA基因文库后,再以计算机仿真的方式,选出能将上述引物扩增的16SrDNA基因中间切割成片段长度范围集中的一组限制性内切酶——AciI、BstUI、和RsaI。结果表明,无施肥土壤与添加堆肥土壤中微生物较接近。无施肥、添加堆肥、添加化肥土壤三者的微生物多样性指数分别为0.990、0.986和0.962;均匀度为0.979、0.977和0.931。
The microbial diversity and evenness in three different soil samples： compost-treated, chemical fertilizer-treated and control group soil samples were studied. The 16S rRNA gene cloning and restriction fragment length polymorphism （RFLP） were used for the comparison of soil microbe community. The optimization of RFLP digestion was firstly carded out, and AciI, BstUI, RsaI were optimal restriction enzymes for the digestion. The diversity indexes of soil microbes were also calculated and the indices of three treatments： control, compost, and chemical fertilizer-treated soils, are 0.990, 0.986, and 0.962 for D value, respectively; and 0.979, 0.977, and 0.931 for evenness, respectively.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第12期2474-2480,共7页
Scientia Agricultura Sinica
关键词
堆肥
化肥
土壤微生物
多样性
均匀度
Compost
Chemical fertilizer
Soil microorganism
Diversity
Evenness
