摘要
目的:了解非小细胞肺癌(NSCLC)组织中基质金属蛋白酶-9(MMP-9)和表皮生长因子受体(EGFR)蛋白的表达及其与肺癌组织类型、细胞分化和淋巴结转移的关系。方法:应用S-P免疫组化的方法检测38例非小细胞肺癌组织和10例正常肺组织中MMP-9和EGFR蛋白的表达。结果:38例NSCLC中MMP-9蛋白的阳性表达率为78·9%(30/38),而中度至强阳性的表达率为60·5%(23/38),与正常对照组比较有显著性差别(P<0·05),其中鳞癌、腺癌MMP-9蛋白的阳性表达率分别为60·0%(12/20)和88·9%(16/18)(P<0·05);有纵隔淋巴结转移组肿瘤组织MMP-9阳性表达率93·3%(28/30),显著高于无纵隔淋巴结转移组50·0%(4/8)(P<0·05);中低分化肿瘤组织MMP-9阳性表达率为80·8%(21/26),明显高于高分化肿瘤组织58·3%(7/12)(P<0·05)。EGFR蛋白在38例NSCLC的表达率为57·9%(22/38),在正常肺组织表达为阴性,二者比较有显著性差异(P<0·05),其中鳞癌、腺癌EGFR蛋白的阳性表达率分别为45·0%(9/20)和72·2%(13/18),二者比较无显著性差异(P>0·05)。有纵隔淋巴结转移组EGFR阳性表达率56·7%(17/30),显著高于无淋巴结转移组37·5%(3/8)(P<0·05);中低分化肿瘤组织MMP-9阳性表达率为69·2%(18/26),明显高于高分化肿瘤组织33·3%(4/12)(P<0·05)。MMP-9和EGFR蛋白在Ⅲ期NSCLC中共同表达率占44·7%(17/38),显著高于Ⅰ期和Ⅱ期患者的13·1%(5/38)。结论:NSCLC中MMP-9蛋白的表达与肺癌的病理分型、细胞分化及淋巴结转移有关;EGFR蛋白的表达和肺癌的TNM分期和淋巴结的转移有关;提示EGFR的信号转导与MMP-9在肿瘤浸润和转移过程中发挥重要作用,MMP-9和EGFR的共同高表达是肺癌不良预后的指标。
Objective ：To investigate the expression and biological significance of MMP-9 and EGFR in 38 NSCLC patients and 10 cases normal lung tissues in order to seek their relationship between them and the prognostic significance. Methods： Tissues were obtained from 38 cases in NSCLC and 10 cases normal lung tissue. The expression of MMP-9 and EGFR were examined by immunohistochemical technique （streptavidin-peroxidase conjugate method, S-P） and the results were correlated with clinical pathological tumor parameters. Results：The positive expression of MMP-9 and EGFR in 38 cases NSCLC were 78.9% （30/38） and 57.9% （22/ 38 ） respectively which was significantly higher than that in normal lung tissues （ P〈0. 05 ）. The positive expression of MMP-9 protein was related with histological classification cell differentiation and also related to lymph node metastasis （ P 〈 0.05 ）, The positive expression of EGFR protein was not related to histological classification, but it was related to lymph node metastasis and cell differentiation （ P〈0. 05 ）. The rate of MMP-9 protein and EGFR protein co-expression in stage Ⅲ NSCLC was 44. 7% （ 17/38 ） , and the expression of M MP-9 and EGFR was significantly higher than that in stage Ⅰ and stage Ⅱ （ 13. 1% ,5/38）. Conclusion： MMP-9 and EGFR may play important roles in NSCLC invasion and metastasis. Over expression of MMP-9 and EGFR protein in NSCLC could act as a reference to evaluate metastasis and unfavourable prognosis of NSCLC.
出处
《临床肿瘤学杂志》
CAS
2005年第6期561-564,共4页
Chinese Clinical Oncology
基金
国家人事部留学回国人员科研启动基金项目(批准号:国人部发[2003]50号)
