摘要
目的:观察川芎嗪对急性脊髓损伤大鼠的体感诱发电位的影响,并与治疗脊髓损伤的西药氢溴酸加兰他敏进行阳性对照。方法:实验于2005-06/09在大连大学整形外科研究所完成。取鼠龄两三个月的健康雄性SD大鼠60只,随机分为3组(n=20):生理盐水组、川芎嗪和氢溴酸加兰他敏组,所有大鼠用AllenWD法(10g×10cm的致伤力)损伤大鼠T8～L1脊髓,各组术后分别腹腔注射5mL生理盐水、川芎嗪(10g/L)、氢溴酸加兰他敏(0.0025g/L),1次/d,持续给药4周。4周后观察各组大鼠体感诱发电位的恢复情况(包括刺激点至L1,T8的潜伏期,L1～T8的传导速度和所用时间),并以20只正常大鼠的体感诱发电位值为正常参考值。结果:80只大鼠进入结果分析。①L1～T8的传导速度:川芎嗪组和氢溴酸加兰他敏组均比生理盐水组快[(46.67±12.74),(45.56±13.33),(22.03±7.17)m/s,P<0.01],但两组间比较无差异,且都比正常组慢。②L1～T8传导所用时间:川芎嗪组和氢溴酸加兰他敏组均比生理盐水组短[(1.39±0.76),(1.42±0.54),(2.87±0.78)ms,P<0.01],但两组间比较无差异,且都比正常组慢。③刺激点至L1,T8的潜伏期:川芎嗪组和氢溴酸加兰他敏组均比生理盐水组短,但未达到正常组大鼠的水平。结论:川芎嗪能有效促进脊髓损伤后神经功能的恢复,改善体感诱发电位各指标,其效果与氢溴酸加兰他敏相似。
AIM： To observe the effect of hgustrazine on somatosensory evoked potential （SEP）of rats with acute spinal cord injury and its positive comparison with galantamin hydrobromide for treatment of spinal cord injury .
METHODS： The experiment was carried out in the Institute of Plastic Surgery, Dalian University from June to September 2005. Sixty male healthy SD rats of 2-3 months old were randomly divided into 3 groups （n=20）：Saline group, ligustrazine group, and galantamin hydrobromide group. T8-L1 spinal cord segments of all rats were injured with Allen WD method （10g×10cm injured power）. Intraperitoneal injection of saline（5 mL）, ligustrazine（10 g/L） and galantamin hydrobromide（0.0025g/L） were administrated in postoperative rats of each group respectively. The administration was given once a day for 4 continuous weeks. After 4 weeks, the recovery of SEP was observed （including the incubation period from exciting point to L1, T2, conduction velocity and time from L1 to T8 ）. SEP of twenty normal rats were taken as normal reference value.
RESULTS： Eighty rats were involved in the analysis of results. （1）Conduction velocity from L1-T8： It was quicker in the ligustrazine group and galantamin hydrobromide group than in the saline group [（46.67±12.74）, （45.56±13.33）, （22.03±7.17）m/s,P〈 0.01], but there was no difference between the former two groups, and both of them were slower than that in the normal group. （2）Conduction time from L1 to T8： It was shorter in ligustrazine group and galantamin hyderbromide group than that in the saline group[（1.39±0.76）, （1.42±0.54）, （2.87±0.78）ms,P 〈 0.01], but there was no difference between the former two groups, and they were slower than that in the normal group. （2）Incubation period from exciting point to L1, T2： It was shorter in the ligustrazine group and galantamin hydrobromide group than in the saline group, and they were still lower than that in the normal group.
CONCLUSION： Ligustrazine can effectively promote the recovery of nerve function following spinal cord injury, and it can also ameliorate every index of SEP.The effect of ligustrazine is similar to the effect of galantamin hydrobromide.
出处
《中国临床康复》
CSCD
北大核心
2005年第45期60-61,共2页
Chinese Journal of Clinical Rehabilitation
