摘要
采用3,5-二硝基水杨酸(DNS)比色法测定麦冬多糖含量。结果表明,麦冬多糖占麦冬总干重的40.4mg.g-1,RSD=2.48%(n=5),总糖供试液和单糖供试液的平均回收率分别是100.4%(RSD=0.17%)和101.1%(RSD=0.62%)。试验表明,DNS比色法测定多糖含量简单易行,且重现性好,可作为麦冬多糖含量的常规测定方法。
3,5-Dinitrosalicylic acid （DNS） colorimetry was used to determine the content of polysaccharide in Ophiopogon japonicus. The result showed that the content of polysaccharide in Ophiopogon japonicus was 40.4 mg·g^-1 with RSD=2.48%（n=5）; average recoveries of total sugar solution and monosaccharide solution in the test were 100.4% with RSD,0.17%（n=5） and 101.1% with RSD,0.62%（n=5） respectively. This method could be used to determine the contents of polysaccharide in Ophiopogon japonicus.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2005年第5期628-630,共3页
Journal of Shenyang Agricultural University
基金
大连市科技攻关项目(2004B3SF175)
关键词
麦冬
多糖
3
5-二硝基水杨酸比色法
Ophiopogon japonicus
polysaccharide
3, 5-dinitrosalicylic acid
