摘要
目的:通过观察联苯双酯(DDB)对四氯化碳(CC l4)所致大鼠急性肝损伤的防治作用,结合文献复习,对DDB的临床应用价值进行再评价。方法:24只大鼠随机分为3组:正常对照组、肝损伤模型组(简称模型组)、DDB治疗组(简称DDB组),每组8只。模型组和DDB组分别用CC l4造成大鼠急性实验性肝损伤,DDB组加用DDB滴丸(5 m g.kg-1)灌胃,每天1次,共14 d,正常对照组和模型组大鼠每天用生理盐水(20 mL.k-g 1)灌胃。实验结束后,检测各组大鼠血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(A ST)的含量,并取肝脏做病理组织检查。结果:DDB组血清ALT、A ST水平较模型组明显降低(P<0.01)。DDB组大鼠肝组织切片中肝细胞脂肪变性、炎细胞浸润均较模型组明显减轻,且肝组织中无明显纤维增生。结论:DDB能显著降低CC l4所致急性肝损伤大鼠血清ALT、A ST水平,对CC l4所致大鼠的肝损伤具有显著的防治作用。
Objective： To observe protective effect of dimethyl diphenyl bicarboxylate（DDB） on the liver with acute injury induced by carbon tetrachloride（CCl4） in rats. Methods： Twenty-four rats were randomly divided into three groups; normal control group, liver injury model group （model group） and DDB treatment group （DDB group）, each group with 8 rats; liver injury of rats was induced by CCl4 in model group and DDB group; the DDB group were simultaneously fed with DDB droplets （5 mg·kg^-1·d^-1）for 14 days, instead of that the normal control group and the model group were fed with physiological salt solution （20 mL·kg^-1·d^-1）for 14 days by the stomach feeding; the serum levels of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） were measured and the liver was pathologically observed after the termination of the experiment. Results： The serum levels of ALT and AST in the DDB group were, significantly lower than those in model group （P〈0.01）; pathologically, DDB group had significantly decreased fatty degenera tion. inflammatory infiltration and fibrosis in the livers than did in model group. Conclusion： DDB could reduce the serum levels of ALT and AST of the rats with the livers injured by CCl4, and had the potential to protect the injured livers of rats.
出处
《广东医学院学报》
2005年第6期651-653,共3页
Journal of Guangdong Medical College
关键词
急性肝损伤
大鼠
联苯双酯
四氯化碳
模型
acute liver injury
rats
dimethyl diphenyl bicarboxylate（DDB）
carbon tetrachloride
animal model
