摘要
为了从分子水平研究狂犬病糖蛋白基因(G)从第4位重排至第1位后病毒的结构、功能及病毒致病性的变化,运用基因突变、反向遗传技术的原理与方法,将狂犬病毒(RV)糖蛋白基因从野生型的第4位(N-P-M-G-L)重排于第1位(G-N-P-M-L),并成功地拯救已发生基因重排的狂犬病毒.
Rabies virus contains nonsegmented negative-sense RNA in its genome. The 11-kb genome encodes five genes in the order 3＇-N-P-M-G-L-5＇ and transcription is obligatorily sequential from the single 3＇ promotor. The G protein plays an important role in viral pathogenicity. Previous studies have demonstrated that moving the N gene to successively more promoter-distal position resulted in stepwise attenuation of replication and lethality for mice. In the present a full-length plasmid with the G gene at promoterproximal position was constructed and a new rabies virus were rescued by reverse genetics.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2006年第1期104-106,共3页
Journal of South China Agricultural University
基金
美国NIH资助项目(051060)
