期刊文献+

呼吸道合胞病毒病原学检测技术的研究进展 被引量:7

Progress of Techniques for Pathogenic Detection of Respiratory Syncytial Virus
原文传递
导出
摘要 呼吸道合胞病毒(RSV)是全球婴幼儿下呼吸道感染最重要的病毒病原,目前检测RSV的技术主要有病毒分离培养、免疫学和分子生物学检测。病毒分离培养技术不适合临床早期诊断和疫情应急诊断的需要;免疫学检测技术具有灵敏和快速的特点,主要有免疫荧光检测技术和酶免疫测定等;分子生物学检测技术主要有核酸分子杂交、PCR和随机扩增多态DNA指纹技术等,特别是PCR技术具有更高的灵敏度和特异性。所有针对RSV病原学的检测技术均有其优缺点,开发快速、灵敏和特异的检测技术是未来RSV病原学检测技术的发展方向。
出处 《国际儿科学杂志》 2006年第1期11-14,共4页 International Journal of Pediatrics
  • 相关文献

参考文献16

  • 1Kehl SC,Henrickson KJ,Hua W,et al.Evaluation of the Hexaplex assay for detection of respiratory viruses in children[J].Clin Microbiol,2001,39(5):1696-1701.
  • 2Landry ML,Ferguson D.Suboptimal detection of influenza virus in adults by the Directigen Flu A+B enzyme immunoassay and correlation of results with the number of antigen-positive cells detected by cytospin immunofluorescence[J].Clin Microbiol,2003,41(7):3407-3409.
  • 3Hu A,Colella M,Tam JS,et al.Simultaneous detection,subgrouping,and quantitation of respiratory syncytial virus A and B by real-time PCR[J].Clin Microbiol,2003,41(1):149-154.
  • 4Reina J,Gonzalez Gardenas M,Ruiz de Gopegui E,et al.Prospective evaluation of a dot-blot enzyme immunoassay(Directigen RSV)for the antigenic detection of respiratory syncytial virus from nasopharyngeal aspirates of paediatric patients[J].Clin Microbiol Infect,2004,10(11):967-971.
  • 5Aldous WK,Gerber K,Taggart EW,et al.A comparison of Binax NOW to viral culture and direct fluorescent assay testing for respiratory syncytial virus[J].Diagn Microbiol Infect Dis,2004,49(4):265-268.
  • 6Ohm-Smith MJ,Nassos PS,Haller BL.Evaluation of the binax NOW,BD directigen,and BD directigen EZ sssays for detection of respiratory syncytial virus[J].Clin Microbiol,2004,42(7):2996-2999.
  • 7Aldous WK,Gerber K,Taggart EW,et al.A comparison of Thermo Electron RSV OIA to viral culture and direct fluorescent assay testing for respiratory syncytial virus[J].Clin Virol,2005,32(3):224-228.
  • 8Slinger R,Milk R,Gaboury I,et al.Evaluation of the QuickLab RSV test,a new rapid lateral-flow immunoassay for detection of respiratory syncytial virus antigen[J].Clin Microbiol,2004,42(8):3731-3733.
  • 9Liolios L,Jennery A,Spelman D,et al.Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens[J].Clin Microbiol,2001,39(8):2779-2783.
  • 10Syrmis MW,Whiley DM,Thomas M,et al.A sensitive,specific,and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples[J].Mol Diagn,2004,6(2):125-131.

同被引文献50

引证文献7

二级引证文献34

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部