摘要
研究了以刚果红分光光度法测定壳聚糖，探讨了最佳实验条件及干扰因素的影响。在pH9．7时，刚果红和壳聚糖形成有色物质，检测波长为543．5nm，线性方程为：A=0．0200ρ＋0．0156，R^2=0．9978。在0～20．00μg／mL范围内呈现良好的线性关系，平均回收率为99．20％。采用该方法测定了两种复方样品中的壳聚糖，方法的选择性和灵敏度良好。该方法可用于检测复方样品中少量的壳聚糖。
A method for the photometric determination of chitosan with Congo Red was studied. The optimal experimental conditions were established. Congo Red can form a colored complex with chitosan at pH 9.7, The maximum absorbanee of the complex is at 543.5 nm. The linear equation is A=0.0200p ＋ 0.0156, R^2 = 0.9978. The linear range is 0 - 20.00μg/mL and the average recovery is 99.20 % . The quantities of chitosan in different health food samples have been determined. The selectivity and sensitivity of this method are satisfactory.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第1期111-113,共3页
Chinese Journal of Analysis Laboratory
