摘要
目的获得能稳定产生竹红菌素的竹黄无性型菌株,通过人工培育获得其活性成分竹红菌甲素。方法通过组织分离,从野生竹黄子座中分离筛选出无性型菌株0258,进行人工条件下的发酵培养。对其发酵产物进行提取、分离和纯化,通过理化性质测定和波谱(UV/V IS、IR、M S)技术进行结构鉴定。结果从竹黄子实体分离筛选到一株无性型菌株0258,其能产生稳定的竹红菌甲素。结论首次获得稳定产生竹红菌素的竹黄无性型菌株,解决了竹黄人工培育的种源问题,使竹红菌素的工业化生产成为可能。
Objective To obtain anamorphosis of Shiraia bambusicola producing the component, hypocrellin A effectively. Methods A strain was isolated from the fruit of S. bambusicola by separating its hypha and was cultured by solid state fermentation. One of the pure chemical compounds isolated from the stroma of wild S. bambusicola was studied on the basis of UV/VIS, IR, MS spectrometric analysis and physicochemical constants. Results The pigment is proved to be hypocrellin A. The isolate was determined as the stable anamorphosis strain 0258 of S. bambusicola. Conclusion A namorphosis strain of S. bambusicola is obtained and precisely appraised for the first time. This study will provide basis for the future massively industrialized hypocrellin production.
出处
《中草药》
CAS
CSCD
北大核心
2006年第1期48-50,共3页
Chinese Traditional and Herbal Drugs
基金
国家技术创新基金资助项目(02CJ-13-01-16)
关键词
竹黄
竹红菌甲素
无性型
波谱分析
Shiraia bambusicola P. Henn
hypocrellin A
anamorphosis
spectrometric analysis
