摘要
目的探讨维生素A缺乏对1,2二甲基肼(DMH)诱导大鼠肾母细胞瘤成瘤的影响。方法建立DMH诱导的Wistar幼鼠肾母细胞瘤动物模型,设立正常饮食组,维生素A缺乏饮食组和对照组,每组各30只。按Beckwith的诊断标准确定肾母细胞瘤和肾源性剩余。观察3组鼠肾母细胞瘤成瘤率和肾源性剩余发生率,流式细胞仪检测肿瘤标本的DNA指数(DI)和S期细胞分布,RTPCR方法检测肿瘤标本RARαmRNA基因表达。结果对照组无肿瘤发生,正常饮食组大鼠成瘤率13.3%,维生素A缺乏饮食组成瘤率36.7%,组间差异有统计学意义(P<0.05)。2组肾源性剩余发生率分别为3.3%和16.7%,差异有统计学意义(P<0.05)。维生素A缺乏饮食组肿瘤DI值1.49±0.22,S期细胞含量(24.1±5.1)%,明显高于正常饮食组的(1.32±0.13)和(15.2±3.6)%。维生素A缺乏饮食组RARαmRNA表达强度(0.18±0.03)明显低于正常饮食实验组(0.49±0.05)。结论维生素A缺乏影响后肾胚基分化、RARαmRNA表达和细胞增殖,导致DMH诱导的大鼠肾母细胞瘤成瘤率增高。
Objective To study the effect and the mechanism of vitamin A deficiency （VAD） on neoplasia of nephroblastoma induced by dimethylhydrazine dihydrochloride （DMH）. Methods A total of 90 Wistar rats weighing 40 -50 g were divided into 3 groups：normal diet （ND） group （n=30） ,VAD group （n =30） and control group （n = 30 ）. In ND and VAD groups, 0.35% DMH was injected subcutaneously and the animals were fed with normal diet in ND group, and with vitamin A deficient diet in VAD group. In control group,the animals were fed with normal diet without DMH. The incidence of nephroblastomas and nephrogenic rests （NRs） in 3 groups were recorded. The renal tissues were prepared for detection of DNA index （DI） and S fraction of cell cycle by FCM ,and for RARer mRNA expression by RT-PCR. The incidence of nephrohlastomas and NRs were determined according to Beckwith diagnostic criteria. Results No tumor developed in control group; the incidence of nephroblastoma in VAD group （36.7%） was significantly higher than that of ND group （ 13.3% ,P 〈0.05 ）. The incidence of NRs in VAD group （ 16.7% ） was also significantly higher than that in ND group （ 3.3% , P 〈 0. 05 ）. The DI and S fraction of tumor in VAD group were 1.49 ±0.22 and 24.1 ± 5.1, which were significantly higher than those in ND group （ 1.32 ± 0.13 and 15.2 ± 3.6 ; P 〈 0.05 and P 〈 0. 001 , respectively）. The expression of RARctmRNA in VAD group （0.18 ± 0.03） was significantly lower than that in ND group （0. 49±0.05, P 〈 0. 001 ）. Conclusions Vitamin A deficiency can increase the incidence of nephroblastomas in the DMH-induced model of rats.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2006年第2期80-82,共3页
Chinese Journal of Urology
基金
国家自然科学基金资助项目(30070780)
