摘要
目的设计、构建日本血吸虫单克隆抗独特型抗体NP30重链(H链)CDR3区6倍重复表位基因(CDR3)6,并对表达产物进行初步鉴定。方法克隆NP30H链(CDR3)6基因,导入PET-28(a)载体并在E.coliBL21中表达;表达产物经纯化后用ELISA方法测定活性。结果(CDR3)6基因被成功构建、表达,表达产物经ELISA检测证实其可与抗NP30抗体NP48及日本血吸虫病人血清反应。结论(CDR3)6部分保留了抗独特型抗体表位的亲和性和特异性。
Objective To design and construct H chain （CDR3）6 gene of anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum, and to characterize the immunological activity of its product. Methods The H chain （CDR3）6 gene of anti idiotypic monoclonal antibody NP30 was cloned into expression vector PET-28（a）. The recombinants was transformed into E. coli BL21 to get expressed, purified and the antigen immunological activity of expressed product was detected by ELISA. Results The H chain （CDR3）6 gene has been successfully constructed, expressed, and its product has been found to react with anti-NP30 antibody （NP48） and schistosomasis patient sera by ELISA. Conclusion The H chain （CDR3）6 fragment of anti-idiotypic monoclonal antibody NP30 could partially keep the affinity and specificity of its parent epitope.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2006年第1期15-18,共4页
Chinese Journal of Schistosomiasis Control
基金
国家高技术研究发展计划(863计划)(2002AA214181)
关键词
日本血吸虫
抗独特型抗体
H链
CDR3
克隆
表达
Schistosoma japonicum
Anti-idiotypic antibody
H chain
CDR3
Cloning
Expression
