摘要
目的探讨TNF-α对人肺癌A549细胞凋亡的影响;方法培养人肺癌A549细胞,与TNF-α(10、100U·ml-1)共孵育,通过Western blot分析P53蛋白表达的变化,同时利用Annexin V/PI双染色流式细胞仪,检测人肺癌A549细胞凋亡率的变化。结果与空白组相比,TNF-α组的P53蛋白表达明显升高,且细胞凋亡率也明显升高,分别为1.22±0.62和1.65±0.38(P<0.01)。结论TNF-α可以诱导人肺癌A549细胞凋亡,从而可能起到抗肿瘤的疗效。
AIM： To observe the effect of TNF-α on the apoptosis of A549 cells. METHODS： A549 cells were cultured and incubated with TNF-α （10, 100 U·ml^-1）. Western blot was used to observe the change of P53 protein, and the cell apoptosis was observed by the Annexin V/PI staining. RESULTS： The expression of P53 protein and the number of apoptotic cells increased significantly after treatment with TNF-α as compared with the control group （P 〈 0.01）. The apoptosis rate of A549 cells was 1.22 ± 0.62 and 1.65 ± 0.38 in the TNF-α group with different dose respectively （P 〈 0.01 ）. CONCLUSIONS： TNF-α can induce the apoptosis of A549 cells and maybe provide the new idea for the therapy of lung cancer.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第12期1381-1383,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
