摘要
以报道较多的蛴螬病原真菌-布氏白僵菌、绿僵菌小孢变种作参照菌,系统研究了新分离鉴定的绿僵菌大孢变种的生物学特性,测定了其在偏低的自然气温下对蛴螬的致病能力.结果表明,绿僵菌大孢变种与参照菌的培养要求接近,最适宜的温度为25℃.3种供试的培养基中,PPDA为最适宜的培养基,其次为SDAY.在偏低的温度环境下绿僵菌大孢变种对蛴螬的毒杀能力强于布氏白僵菌,土壤和喷雾处理后累计僵虫率分别达88.23%和76.47%,有很好的田间应用前景.
With Beauveria brongniartii and Metarhizium anisopliae var. anisopliae as the reference, this paper studied the biological characteristics and invading ability of Metarhizium anisopliae var. major, a new variety separated from white grubs. The results showed that the optimal culture condition of M. anisopliae vat. major was similar with that of other entomopathogenic species, and the optimal temperature was 25℃. Among the three test media, PPDA was the most appropriate one, followed by SDAY and Czapek. M. anisopliae var. najor had a stronger virulence than B. brongniartli at lower temperature, with an accumulative mortality of white grubs being 88. 23 % and 76.47 %, respectively, after treated with soil and through spraying, suggesting that M. anisopliae vat. major had a good prospect to widely apply in field.
出处
《应用生态学报》
CAS
CSCD
北大核心
2006年第2期351-353,共3页
Chinese Journal of Applied Ecology
基金
国家自然科学基金重大项目(30330500)
安徽省学术带头人资助项目(2002HBL17).
关键词
金龟子绿僵菌
布氏白僵菌
生物学特征
蛴螬
毒力
Metarhizium anisopliae, Beauveria brongniartii, Biological characteristics, White grub, Virulence.
