摘要
目的探讨黄芪活性成分黄芪甲甙对病毒性心肌炎(VM)小鼠心肌中神经生长因子(NGF)表达的影响。方法Balb/c小鼠100只,随机分成6组。非感染小鼠腹腔无菌注射病毒培养液,分为正常对照组[A组,10只,以羧甲基纤维素钠(CMC)0.1 mL灌胃7 d]、高剂量对照组(B组,10只,9%黄芪甲甙液0.1 mL灌胃7 d);余80只小鼠以柯萨奇病毒(CVB3)腹腔无菌注射制作VM模型,VM小鼠随机分为心肌炎对照组和低、中、高剂量干预组,分别以CMC、1%、3%、9%黄芪甲甙液0.1 mL灌胃7 d(分别为C、D、E、F组,每组20只)。14 d后处死小鼠并取其心脏,采用RT-PCR和免疫组织化学检测NGF mRNA及蛋白表达水平。结果F组小鼠较C组死亡率明显降低,B组无小鼠死亡;与C组比较,NGF mRNA、蛋白表达水平以及心肌病变积分在F组均显著下降,而低、中剂量黄芪甲甙对心肌炎小鼠NGF mRNA、蛋白表达及心肌病变积分无明显影响。结论黄芪甲甙可能通过抑制NGF表达,对Balb/c小鼠CVB3心肌炎具有良好的治疗作用。
Objective To investigate the effect of astragaloside, one of the active components of astragalus membranaeeus, on myocardial nerve growth factor（NGF） expression in murine myocarditis model with eoxsackievirus B （CVB3）. Methods One hundred Balb/c mice were randomly divided into 6 groups. （;roup A [ n = 10, treated with carboxymethylcellulose（CMC） 0.1 mL, ig, for 1 week] were served as normal controls group and group B （ n - 10, treated with 9 % astragaloside 0. 1 mL, ig, for 1 week）as high - dose control. Eighty infected mice treated with （TMC and 1% ,3% ,9% astragaloside 0.1mL,ig, for I week （ n - 20 in each group）were served as group C （myoearditic control group）, D （low dose intervention group）, E （middle- dose intervention group）, F （high- dose intervention group）, respectively. The mice were killed and their hearts were removed after 14 days. The expression levels of NGF mRNA and protein in the myocardium were examined by RT PCR and immunohistochemistry, respectively. Results The mortality was significantly re duced in 9% astragaloside treated infected mice that was 10% （2/20） v.; 45% （9/20）in group C （P（0.05）. In uninfected miee,9% astragaloside did not cause any death. The expreasion levels of NGF mRNA, protein and myocardial hlstopathologie scores were significantly lower in group F than those in group C. However, the NGF expression and myocardial histopathologic scores were not affected by 1% and 3% astragaloside. Conclusion Astragaloside might play a pivotal role in protecting the heart injure in Balb/c mice myocarditis with CVB3 by sunDreasing excessive expression of NGF.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第1期14-16,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(30271665)
