摘要
目的:观察体外培养大鼠骨骺干细胞及其生长特性。方法:采用显微取材后酶消化获取骨骺干细胞,用Percoll密度梯度离心法纯化,并测定其细胞生长曲线、细胞分裂指数、碱性磷酸酶活性,用免疫组化及免疫荧光染色的方法检测Ⅱ、Ⅹ型胶原的合成情况和透射电镜观察粗面内质网的丰富程度。结果:大鼠骨骺干细胞形状类似成纤维细胞,呈梭型,碱性磷酸酶活性低,粗面内质网少,Ⅱ型胶原合成量较多。结论:体外培养的骨骺干细胞能向成熟阶段分化,可为软骨或骨组织工程提供理想的种子细胞。
Objective： To study the culture of mouse epiphysis stem cells in vitro and the growth characteristics for the actual application. Methods： Epiphysis stem cells were harvested by collagenase digestion after microoperation, and purified by Percoll density gradient centrifugation, then the cell growth curve, mitotic index and alkaline phosphatase were observed, the types Ⅱ and Ⅹ collagen were detected by immunohistochemistry and immunofluorescence, and the transmission electron microscope was used to observe the content of rough endoplasmic reticulum. Results： Mouse epiphysis stem cells were fibroblast-like, with little alkaline phosphatase and rough endoplasmic reticulum, and much type 1I collagen. Conclusion： Epiphysis stem cells cultured in vitro can differentiate into the mature cells, which could provide ideal seeding cells for the cartilage or bone tissue engineering.
出处
《中国康复》
2006年第1期3-5,共3页
Chinese Journal of Rehabilitation
基金
国家自然科学基金资助项目(30371439)
关键词
骨骺干细胞
细胞培养
细胞分化
epiphysis stem cells, cell culture, cell differentiation
