摘要
目的：探讨红色毛癣菌对抗真菌药物的敏感性与其种株特异性之间的关系。方法：采用微量稀释法，检测红色毛癣菌对7种临床常用抗真菌药物的敏感性，并对红色毛癣菌的基因型、表型、分离部位等与对抗真菌药物的敏感性进行相关性研究。结果：不同基因型、表型及不同部位分离的红色毛癣菌菌株对特比萘芬、萘替芬、伊曲康唑的MIC值范围较集中（分别为0．016～0．032、0．032～0．063、0．25～1μg／ml），且前两者的MIC值更小（众数均为0．032μg／ml），而对酮康唑和氟康唑的MIC值范围则相差较大（分别为0．25～2、1～32μg／ml）。Wilcoxon秩和检验显示，红色毛癣菌对特比萘芬、萘替芬、伊曲康唑、两性霉素B等4种抗真菌药物的MIC与基因型、表型、分离部位的不同无显著相关。结论：红色毛癣菌对抗真菌药物的敏感性可能与基因型、表型或分离部位无关。
Objective：To study the relationship between susceptibilities of Trichophyton rubrum strains to antifungal agents and their species specificities. Methods： The susceptibilities of Trichophyton rubrum strains to itraconazole, ketoconazole, fluconazole, terbinafine, naftifine, 5-flucytosine and amphotericin B were evaluated using a modified microdilution method. The relationship between susceptibilities and genotypes and phenotypes of Trichophyton rubrum strains with different origins was analyzed by random amplified polymorphic DNA （RAPD）. Resultss The Trichophyton rubrum strains showed narrow minimum inhibitory concentration （MIC） ranges to terbinafine（0. 016-0. 032μg/ml）, naftifine（0. 032-0. 063μg/ml） and itraconazole（0.25- 1μg/ml）, whereas they showed broader MIC ranges to ketoconzole（0.25-2 μg/ml）and fluconazole（1-32 μg/ml）. MICs of Trichophyton rubrum strains to terbinafine （M0 = 0. 032μg/ml） and naftifine （M0 = 0. 032μg/ml） were the lowest among 7 antifungal agents. Wilcoxon test （Kruskal-Wallis test） suggested that there was no significant relationship of MICs to terbinafine, naftifine, itraconazole and amphotericin B with the genotypes, phenotypes and origins of the Trichophyton rubrum strains. Conclusion：The antifungal susceptibility of Trichophyton rubrum strains may not be related to their genotypes, phenotypes or from which part of the body they are isolated.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第2期136-139,共4页
Academic Journal of Second Military Medical University
关键词
红色毛癣菌
基因型
表型
敏感性
抗真菌药
Trichophyton rubrum
genotype
phenotype； susceptibility
antifungal agent
