摘要
目的探讨N-乙酰半胱氨酸(NAC)对重症急性胰腺炎(SAP)的治疗作用及作用机制。方法54只SD大鼠随机分为假手术(SO)组、SAP组及NAC处理组。采用胰胆管逆行注射5%牛磺胆酸钠诱导大鼠SAP模型,NAC处理组于模型制作前30 min腹腔内注射NAC(200 mg/kg)进行预处理。分别观察各组大鼠血浆淀粉酶、TNF-α、IL-6、丙二醛(MDA)、胰腺及肺组织病理以及组织髓过氧化物酶(MPO)的变化。结果SAP组血浆淀粉酶、TNF-α、IL-6、MDA、胰腺及肺组织MPO均较SO组明显升高(P<0.05),NAC处理组各检测指标均较SAP组显著降低(P<0.05),胰腺及肺组织病理损伤明显减轻。结论NAC可通过清除氧自由基、减少TNF-α和IL-6的产生等机制对SAP具有重要的治疗和保护作用。
Objective To investigate the therapeutic effect and mechanism of N-acetylcySteine （NAC） on severe acute pancreatitis（SAP）. Methods Fifty-four SD rats were randomized into sham operation（SO） group, SAP group and NAC-pretreated group. The model of SAP was induced by retrograde injection of 5% sodium taurocholate into the bill-pancreatic duct. NAC-pretreated group was given 200 mg/kg NAC intraperitoneally 30 rain before inducing SAP. The levels of amylase, TNF-α, iL-6,malondiaLdehyde（MDA） in plasm, and myeloperoxidase（MPO） in the pancreas and lung were measured. The histopathological changes of pancreatic and pulmonary tissues were evaluated. Results The levels of amylase,TNF-α, IL-6, MDA in plasm, and the MPO in the pancreas and lung were significantly increased in SAP group compared with those in SO group（P〈0.05）. Compared with SAP group, the indexes were significantly decreased in NAC-pretreated group（P〈0.05）, the severity of pancreas and lung injury also significantly decreased in NAC-pretreated group. Conclusion NAC may have a therapeutic effect on SAP by cleaning oxygen free radicals and decreasing the production of TNF-α and IL-6.
出处
《江苏医药》
CAS
CSCD
北大核心
2006年第3期248-250,F0002,共4页
Jiangsu Medical Journal
