摘要
以茄子黄萎病菌毒素为选择剂,结合组织培养技术,离体筛选茄子抗黄萎病突变体,开拓一条体细胞抗病育种的新途径。结果表明,以茎段为外植体,在MS+IAA 1 mg/kg+NAA 1 mg/kg+KT 0.2 mg/kg培养基上愈伤组织诱导率最高,下胚轴次之,子叶最差;15%的毒素浓度(v/v)可以作为突变体的筛选压力;逐步提高毒素浓度能够在一定程度上提高愈伤组织对毒素的抗性。
In vitro screening of resistant mutants of eggplant to Verticillium daliae was studied with Verticillium daliae toxin and tissue culture technology. The induction rate in stem on medium of MS medium ＋ IAA 1 mg/kg ＋ NAA 1 mg/kg ＋ KT 0.2 mg/kg was higher than that of in hypocotyl and cotyledons. The toxin concentration of 15 % was considered as the selection pressure of resistant mutants of eggplant to Verticillium dahliae. The resistance of callus to Verticillium dahliae toxin could be improved by increasing the concentration gradually.
出处
《华北农学报》
CSCD
北大核心
2006年第1期92-95,共4页
Acta Agriculturae Boreali-Sinica
基金
内蒙古自然科学基金资助项目(20001303)
关键词
茄子黄萎病菌
毒素
离体筛选
抗病突变体
Verticillium daliae
Toxin
In vitro screening
Resistant mutant
