摘要
目的:建立同时测定虎杖及其提取物中白藜芦、大黄素、大黄酚、大黄素甲醚4种有效成分含量的高效液相色谱法。方法:Hypersil C18色谱柱(4.6 mm×200 mm,5μm),流动相为乙腈和1%HAc水溶液,梯度洗脱,流速1 mL.min-1,检测波长287 nm。结果:方法的平均回收率及RSD分别为:白藜芦醇101.8%,1.7%;大黄素96.2%,1.4%;大黄酚99.7%,1.0%;大黄素甲醚98.6%,0.97%。结论:该方法可用于虎杖药材及其提取物的质量控制。
Objective： to establish a HPLC method for simultaneous determination of resveratrol, emodin, chrysophanol, physcion in root of Polygonum cuspidatum and its extract. Method： The separation was performed in a Hypersil ODS C18column with a mobile phase of Acetonitrile-1% HAc. The flow rate was 1.0 mL·min^-1 and detection wavelength was at 287 nm. Result： The mean recovery and RSD of resveratrol, emodin, chrysophanol, physcion was 101.8 %, RSD 1.7 % ; 96.2 %, RSD 1.4 % ： 99.7 %, RSD 1.0 % ; 98.60 %, RSD 0.97 % respectively. Conclusion： The method is accurate, simple and reliable. It can be used for quality control of P. cuspidatum and its extract.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第3期202-205,共4页
China Journal of Chinese Materia Medica
基金
国家"十五"重大专项基金
湖南省杰出青年基金(03JJY1002
2001BA746C
2001BA804A21
2003AA2Z3515)
