摘要
目的对2株人类免疫缺陷病毒1型(HIV-1)CRF07-BC亚型病毒的完整gag基因和部分pol基因进行基因重组和耐药位点突变分析。方法从确诊的HIV感染者的全血样本中提取基因组DNA,经套式聚合酶链反应(Nested-PCR)扩增后,将扩增产物进行纯化和测序,然后将所得序列进行系统进化树和氨基酸变异分析。用Simplot软件进行序列重组分析以确定重组断点区域,并分析其pol基因的耐药位点突变。结果在所研究的基因区段内,2份样本均未发现重组断点的变化,在蛋白酶区(PR)出现2个次要耐药相关突变,而未发现主要耐药相关突变。结论所研究的2株HIV-1CRF07-BC亚型病毒与在我国新疆地区广泛流行的CRF07-BC模式毒株非常接近,且不存在天然耐药的情况,适于抗逆转录类药物的应用。
Objective To analyze the sequence recombinant and drug resistance mutations of full length gag gene and partial pol gene of 2 Chinese prevalent HIV 1 CRF07-BC subtype strains. Methods Samples of total DNA were extracted from the peripheral blood mononuclear cells to undergo nested PCR and sequencing. The sequences thus obtained underwent phylogenetic tree analysis and amino acids variation analysis, Simplot software was used to analyze the sequence recomoinant and identify the breakpoints of B/C recombinant strains,and analysis of genotypic drug-resistance mutations was performed, Results No change of breakpoints was found in 2 samples in our test,2 secondary drug-resistance mutations were presented in protease gene, and no primary drug-resistance mutations were observed.Conclusions Sequences of 2 samples in our test were similar to some sequences of HIV-1 strains prevalent in Xinjiang region. No native drug-resistance were found. Reverse transcriptase inhibitors were suitable for antiretroviral therapy for these HIV infected persons,
出处
《中国艾滋病性病》
CAS
2006年第1期7-9,共3页
Chinese Journal of Aids & STD
