摘要
目的:观察逆灸关元穴对更年期大鼠下丘脑和血液中应激激素及下丘脑室旁核雌激素受体的影响,探究逆灸对更年期大鼠应激影响及中枢雌激素受体α调节机制。方法:实验于2003-11/2004-06在北京中医药大学针灸学院针灸机理实验室完成。①选用健康SD雌性大鼠128只。其中,4月龄(年轻)16只为年轻正常组。随机将10月龄大鼠112只分为7组:12,14,16月龄逆灸组;10,12,14,16月龄对照组;每组16只。自然老化鼠。所有逆灸组大鼠均在10月龄开始艾炷灸,将艾炷点燃后,直接放置在关元穴上施灸,每次灸一壮。灸后局部皮肤略潮红。1周灸2次,共灸8周。10,12,14,16月龄对照组及年轻正常组除轻抓仰躺外不做其他处理。②每组取10只采用放射免疫法测定下丘脑促肾上腺激素释放激素、血浆促肾上腺激素水平。每组取其余6只采用免疫组化法测定下丘脑雌激素受体α含量。③组间计量资料差异比较采用单因素方差分析。结果:大鼠128只均进入结果分析。①下丘脑室旁核雌激素受体α表达:各模型对照组随增龄,呈波动上升趋势,14和16月龄对照组明显高于年轻正常组(P<0.05,0.01)。各逆灸组均低于同月龄对照组,其中逆灸16月龄组明显低于16月龄对照组(P<0.05)。②下丘脑促肾上腺激素释放激素含量:模型对照各组随增龄呈波动下降趋势,14和16月龄组明显低于年轻正常组(P<0.05～0.01)。逆灸14月龄组明显低于同龄对照组(P<0.05)。③血浆促肾上腺激素水平:模型对照和逆灸各组随增龄升高,14和16月龄组明显高于年轻正常组(P<0.01)。逆灸12和16月龄与同月龄对照组相近(P>0.05),逆灸14月龄组明显低于同月龄对照组(P<0.05)。结论:随增龄更年期大鼠处在一种应激紊乱与失调状态,逆灸可使随后12,14,16月龄大鼠紊乱的应激激素得到一定的调整,并这种作用可能与逆灸调节下丘脑雌激素受体α表达有关。
AIM： To explode effect of moxibustion at Guangyuan point on hypothalami, stress hormone in blood, estrogen receptor of thalamic paraventrieular nucleus, stress and regulating mechanism of central estrogen receptor-α of preventive moxibution in rats during natural menopause. METHODS： The experiment was completed at the Laboratory of Acupuncture and Moxibustion, Beijiug University of Traditional Chinese Medicine from November 2003 to June 2004. ① Totally 128 healthy female SD rats were selected. Sixteen rats aged 4 months were regarded as normal group, and other 112 rats age 10 months were divided randomly into 7 groups： 12-month, 14-month and 16-month moxibution groups, and 12-month, 14-month and 16-month control groups with 16 in each group. All rats in moxibution groups were treated with moxa cone moxibution on Guangyuan poiot once, After rooxibution, skin was a little flush. Moxibution was performed twice a week for 8 weeks. Rats in control groups and normal group were not treated with any method except light catching and lying. ② Each 10 rats were selected from three groups to measure levels of cortieotropin-releasing hormone （CRH） in hypothalami and adrenocorticotrophic hormone（ACTH） in plasma with radio-immunity method. Another 6 rats in each group were selected to assay content of estrogen receptor-α in hypothalami with iromunohistochemistry. ③ Measurement data were compared with one-way analysis of variance among groups. RESULTS; Totally 128 rats entered the final analysis. ① Expression of estrogen receptor-α in thalamic paravcntricular nucleus： Along with ages increasing, the content was also increased, and that in 14 and 16-month control group was higher than that in normal group （P 〈 0.05, 0.01）; while thai in moxibution groups was all lower than that in control groups, especially that in 16-month moxibution group was lower than that in 16- month control group （P 〈 0.05）. ② Content of CRH in hypothalami： Contents in mudel group were decreased, and those in 14- and 16-month groups were lower than those in normal group （P 〈 0.05-0.01）. Content in 14-month moxibution group was lower than that in 14-month control group （P 〈 0.05）. ③ Level of ACTH in plasma： With age increasing, levels in model group and moxibution groups were also increased, and that in 14- and 16-month group was higher than that in normal group （P 〈 0.01）. Levels in 12 and 16-month moxibution groups were similar to those in control group （P 〉 0.05）, and that in 14-month moxibution group was lower than that in 14- month control group （P 〈 0.05）. CONCLUSION： With age increasing, rats during their menopause were in a state of stress disorder and disfunction. Moxibntion can regulate disharmonized stress hormone in a certain extent at the age of 12, 14 and 16 months seqaentially, and this effect may relate to the regulating expression of hypothalami estrogen receptor-α.
出处
《中国临床康复》
CSCD
北大核心
2006年第7期118-120,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金课题(重大研究计划)(90209026)
北京市自然基金课题(7052037)~~
