摘要
目的:研究依他酸(EA)和卡西霉素(Cal)对人胚肺成纤维细胞脱氧核糖核酸和胶原合成的影响。方法:采用[~3H]TdR与[~3H]脯氨酸掺入分别测定脱氧核糖核酸和胶原合成。结果:给药24h后,EA0.05—4mmol·L^(-1)和Cal0.25—20μmol·L^(-1)组胶原合成均显著增加,脱氧核糖核酸合成无明显改变。给药36和48h,EA 1—4mmol·L^(-1)组胶原与脱氧核糖核酸合成降低:Cal0.25—20μmol·L^(-1)组脱氧核糖核酸合成亦下降,胶原合成仅10和20μmol·L^(-1)组降低。结论:细胞外液Ca^(2+)内流促进胶原合成,细胞内Ca^(2+)浓度过高或过低均抑制脱氧核糖核酸合成。
AIM: To study the effects of egtazic acid (EA) and calcimycin (Cal) on the synthesis of DNA and collagen in cultured human lung fibroblasts (HLF). METHODS: The synthesis of DNA and collagen was determined by measuring the incorporation of [3H]TdR and [3H]proline of HLF respectively. RESULTS: The collagen synthesis increased markedly 24 h after exposure to both EA (0.05 - 4 mmol · L-1) and Cal (0.25 - 20 μmol · L-1) , and that there was no obvious change in DNA synthesis. After 36 - 48-h exposure , both DNA and collagen syntheses decreased in the groups of EA (1,2. and 4 mmol · L-1);the DNA synthesis was also suppressed in Cal groups in a concentration-dependent manner, whereas collagen synthesis decreased only in Cal (10 and 20 μmol · L-1). CONCLUSION: Extracellular Ca2+ influx into fibroblasts increased collagen production, However, the DNA synthesis was suppressed when the cytosolic Ca2+ was too high or too low.
出处
《中国药理学报》
CSCD
1996年第1期63-65,共3页
Acta Pharmacologica Sinica
