摘要
目的:采用RNA干扰(RNA interference,RNA i)技术,利用构建的稳定转染CD147 shRNA表达质粒载体的HO-8910pm/pGE-1 CD147shRNA细胞株观察卵巢癌细胞系HO-8910pm CD147基因的沉默作用及生物学效应。方法:用免疫细胞化学法、激光共聚焦检测HO-8910pm细胞CD147的表达;MTT法绘制细胞生长曲线,观察裸鼠卵巢癌皮下移植瘤的成瘤性。结果:pGE-1CD147shRNA转染人卵巢癌细胞系后,CD147的表达受到高效且特异的抑制,细胞侵袭力减弱,荷瘤裸鼠成瘤力下降,抑瘤率达58.8%(P<0.01)。结论:RNA i沉默CD147基因可影响卵巢癌的侵袭和成瘤,有可能成为治疗卵巢癌的新途径。
Objective：To study the effect of CD147 gene silence by RNAi in ovarian cancer cell line HO-8910pm on biological behavior. Methods： Immunohistochemical technique and confocal laser scanning microscope （CLSM） were carried out to assess the expression of CD147 in HO-8910pm cell line. MTT assay was used to observe the effect on proliferation ability;in vitro invasion assay was performed to assess changes of tumor invasion ability. Cells were transplanted subcutaneously in nude mice to observe the tumorigenesis. Results： In HO- 8910pm/pGE-1 CD147shRNA cell ,the expression of CD147 was inhibited specifically and efficiently;no statistical difference of their proliferation abilities was observed but their invasion abilities and tumorigenesis in nude mice decreased significantly. Tumorigenicity inhibition rate reached 58.8% （ P 〈0.01 ） in experimental group. Conclusion： Silencing of CD147 by RNAi can inhibit invasion and tumorigenesis of ovarian cancer and may become a new approach of ovarian cancer therapy.
出处
《现代妇产科进展》
CSCD
北大核心
2006年第2期92-95,F0003,共5页
Progress in Obstetrics and Gynecology
