摘要
目的:探讨女性压力性尿失禁(SUI)患者阴道壁结缔组织中基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶组织抑制物-2(TIMP-2)mRNA表达与SUI发病的关系。方法:7例无SUI妇女为对照组(A组),24例有完整随访资料的女性SUI患者的阴道壁结缔组织标本为研究组,分为<60岁组(B组)和>60岁组(C组)。应用逆转录-聚合酶链反应分别检测MMP-2和TIMP-2表达量。结果:SUI组患者的MMP-2均较非SUI组显著升高,差别有显著性(P<0.01),TIMP-2均显著下降,差异有统计学意义(P<0.01)。MMP-2在B组和C组之间的差异无显著性(P>0.05),TIMP-2的mRNA表达量C组较B组显著下降,差别有统计学意义(P<0.01)。MMP-2/TIMP-2从A组到B组再到C组显著升高,A组<B组<C组,差异有统计学意义(P<0.01)。结论:MMP-2升高,对胶原纤维的降解增加;TIMP-2下降,对胶原纤维降解的抑制作用减弱,MMP-2/TIMP-2升高,使得总体上对胶原纤维的降解增强,可能在SUI的发生过程中起重要作用。
Objective ： To detect the expressions of matrix metalloproteinase-2 （ MMP-2 ） and tissue inhibitor of matrix metalloproteinase-2 （TIMP-2） in the vaginal wall tissue of the women with stress urinary incontinence （SUI） and their significance. Methods：Vaginal wall tissues were obtained from 7 women undergoing total hysterectomy for carcinoma in situ of cervix without SUI as control group（ Group A）. 24 patients with SUI were followed-up as study group which was divided into two groups[ 12 cases were 〈 60 years old （Group B）and 12 cases 〉60 years old（ Group C） ]. Semi-quantitative competitive RT-PCR was carried out to quantify MMP- 2 and TIMP-2 mRNA expression. Results： MMP-2 was significantly higher （ P 〈 0.01 ） in both Group B and Group C than in Group A（ P 〈0.01 ）. There was no difference between Group B and Group C. TIMP-2 was significantly lower（ P 〈0.01 ）in both Group B and Group C than in Group A（P 〈0. 01 ） ,and TIMP-2 in Group C was more significantly lower than in Group B（ P 〈0. 01 ）. The ratio of MMP-2/TIMP-2 was significantly different （ P 〈 0.01 ） among three groups, Group A 〈 Group B 〈 Group C. Conclusion： The increased ratio of MMP-2/TIMP-2 may play an important role in the development of SUI.
出处
《现代妇产科进展》
CSCD
北大核心
2006年第2期114-116,120,共4页
Progress in Obstetrics and Gynecology
基金
福建省科技攻关重点项目(2003Y016)
南京军区福州总医院博士后课题基金资助项目
关键词
尿失禁
压力性
基质金属蛋白酶-2
金属蛋白酶2组织抑制剂
逆转录聚合酶链反应
Urinary incontinence,stress
Matrix metalloproteinase-2
Tissue inhibitors of metalloproteinase-2
Reverse transeriptase-polymerase chain reaction
