摘要
目的:观察成年小鼠缺氧后脑内一氧化氮含量的变化及黄体酮的干预作用。方法:实验于2002-09/2003-06在新乡医学院生理学与神经生物学教研室完成。32只雄性昆明种小鼠,随机分为4组,对照组、单纯缺氧组、低孕酮(4mg/kg)组和高孕酮(8mg/kg)组,后两组于缺氧前30min腹腔注射孕酮4mg/kg或8mg/kg。小鼠缺氧24h断头取脑,检测脑皮质及海马组织中的一氧化氮含量。结果:32只小鼠均进入结果分析。①缺氧24h后,单纯缺氧组脑皮质一氧化氮的生成量明显高于对照组眼(2.15±0.29),(1.66±0.65)μmol/g,P<0.05演;经孕酮预处理的两组,脑皮质组织一氧化氮含量较单纯缺氧组均有所下降,4mg/kg组的效果尤为明显,下降了35.81%(P<0.01)。②海马组织在缺氧24h后,单纯缺氧组一氧化氮含量明显高于对照组眼(1.76±0.60),(1.21±0.58)μmol/g,P<0.05演;低孕酮(4mg/kg)组眼(1.07±0.33)μmol/g演显著低于单纯缺氧组(P<0.01);而高孕酮(8mg/kg)组眼(1.27±0.41)μmol/g演与单纯缺氧组差异无显著性(P>0.05)。结论:成年小鼠缺氧24h后脑皮质和海马组织一氧化氮含量明显升高,黄体酮通过降低这种一氧化氮的升高,可能产生一定的神经保护作用。
AIM： To observe the changes of nitric oxide in the hypoxic cerebral tissues in adult mice and investigate the neuroprotective role of progesterone （PROG） in the cerebral anoxia. METHODS： The experiment was conducted in the Staff Room of Physiology and Neurobiology, Xinxiang Medical College from September 2002 to June 2003. Thirty-two male Kunming mice were divided randomly into control group, simple hypoxia group, lower progesterone （4 mg/kg） group and higher progesterone （8 mg/kg） group. Progesterone was injected intraperitoneally with 4 mg/kg or 8 mg/kg, respectively at 30 minutes before hypoxia in the last two groups. The content of nitric oxide （NO） in cortex and hippocampus in mice were evaluated 24-hours after cerebral anoxia. RESULTS： A total of 32 mice were involved in the result analysis. ① Cortex NO production in simple hypoxia group was significantly higher than that in control group [（2.15±0.29）, （1.66±0.65）μmol/g,P 〈 0.05] at 24-hours after cerebral anoxia. Cortex NO contents in the two groups preconditioning with progesterone were all decreased than that in simple hypoxia group, especially that in lower progesterone group （4 mg/kg PROG） breakdown 35.8% （P 〈 0.01）. ②At 24-hours after cerebral anoxia, the hippocampus NO contents of the simple hypoxia group also rose significantly as compared with that of control group [（1.76±0.60）, （ 1.21±0.58）μmol/g,P 〈 0.05]. The increase of NO of lower progesterone group （4 mg/kg PROG） [（1.07±0.33）μmol/g] also was decreased distinctly as compared with that of simple hypoxia group （P 〈 0.01）. The hippocampus NO contents of higher progesterone group （8 mg/kg PROG） [（1.27±0.41 ）μmol/g] rose insignificantly （P 〉 0.05） as compared with that of simple hypoxia group （P 〉 0.05）. CONCLUSION： At 24-hours after cerebral anoxia, cortex and hippocampus NO contents increased significantly, the progesterone may produce neuroprotective role by inhibiting those rise.
出处
《中国临床康复》
CSCD
北大核心
2006年第12期71-73,共3页
Chinese Journal of Clinical Rehabilitation
基金
河南省自然科学基金(984021100)
河南省医学科技创新人才工程项目资助(24)~~
