摘要
提取堆型艾美耳球虫子孢子杂交瘤细胞总RNA,进行RT PCR,扩增出重链可变区基因。将已扩增出的鸡堆型艾美耳球虫特异性单抗重链可变区基因片段与pMD 18T载体连接,重组载体转化于感受态细胞JM109,筛选出阳性重组子,提取阳性重组子质粒并进行测序,得到单抗重链可变区的基因序列,为单链抗体基因的构建及免疫毒素的构建奠定了基础。
To study the function of small molecule antibody , hight chain variable genes which was amplified from Species - specific Monoclonal Antibodies against E. acervulina was purified ,and Cloned into clone vector pMD18 - T by gene recombination techniques, The recombinant plasmid was transformed into E. coli JM109. By ,screening, several recombinants that had been inserted with the target fragments were obtained. The positive clones containing recombination were idendified by the methods of the gene sequenceing, The resultdemonstrated that hight chain variable genes is 381 bp.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2006年第3期11-13,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
河北省自然科学基金资助项目(303226)
关键词
鸡
堆型艾美耳球虫
单抗
可变区
Chieken
E, acervulina
monoelonal antibodies
variable genes
