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磺胺间甲氧嘧啶全抗原的制备研究 被引量:2

Study on Preparation of Complete Antigen of Sulfamonomethoxine
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摘要 采用重氮化法,将半抗原磺胺间甲氧嘧啶(SMM)与载体牛血清白蛋白(BSA)和卵清白蛋白(OVA)偶联,制得磺胺间甲氧嘧啶全抗原SMM-BSA和SMM-OVA。通过其紫外扫描光谱、SDS-PAGE凝胶电泳试验,表明半抗原和载体成功偶联,免疫动物以后可以产生满意效价。这为进一步制备抗SMM抗体提供了良好的免疫原。 Semiantigen sulfamonomethoxine (SMM) was coupled with carrier protein bovine serum albumin (BSA) and ovalbumin to prepare complete antigen SMM - BSA and SMM - OVA by the method of diazotization. UV scanning spectrum, SDS- PAGE and animal immunity all indicated that the complete antigens both SMM - BSA and SMM - OVA were successfully produced. The present research supplied an excellent immune antigen for further preparation of antibody to SMM.
出处 《河南农业科学》 CSCD 北大核心 2006年第3期96-100,共5页 Journal of Henan Agricultural Sciences
基金 "十五"国家重大科技专项"食品安全关键技术综合示范"(2001BA804A30-08)
关键词 磺胺间甲氧嘧啶 全抗原 紫外扫描 免疫 鉴定 Sulfamethoxydiazine Complete antigen UV scanning spectrum Immunity Identify
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参考文献12

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同被引文献23

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