摘要
目的研究快速眼动(REM)睡眠剥夺和睡眠恢复对大鼠海马及皮质区神经元型一氧化氮合酶(nNOS)转录水平表达的影响。方法将大鼠分为两组,分别为REM睡眠剥夺组(SD组)和对照组(空白对照组:CC组和环境对照组:TC组)采用改良多平台睡眠剥夺法建立大鼠REM睡眠剥夺模型,获取海马及皮质组织,采用半定量逆转录-聚合酶链式反应(RT-PCR),测定REM睡眠剥夺13、、5及7天、恢复睡眠6、12、24及48 h大鼠皮质、海马nNOS mRNA的表达。结果CC组和TC组海马及皮质nNOS mRNA表达无显著性差异。REM睡眠剥夺1天和3天组nNOS mRNA表达增多,睡眠剥夺5天组nNOS mRNA表达明显升高,睡眠剥夺7天组nNOS mRNA达到峰值(0.691±0.008,P<0.01),睡眠恢复后6 h、12 h和24 h组nNOS mRNA表达仍高于TC组。结论大鼠REM睡眠剥夺增加皮质、海马nNOS mRNA的表达。
Objective To study the effects of REM sleep deprivation and recovery on the expression of mRNA coding neuronal nitric oxide synthase （nNOS） of hippocampus and cortex in rats. Methods The modified multiple platform technique was adopted to set up the model of REM sleep deprivation of S-D rats and to take the tissue of hippocampus and cortex. Semiquantitative reverse-transcription-polymerase chain reaction was used to determine the expression of mRNA of nNOS in hippocampus and cortex in rats experiencing REM sleep deprivation for 1 d, 3 d, 5 d,7 d and recovery for 6 h, 12 h, 24 h, 48 h. Results The expression of nNOS mRNA in rats＇ hippocampus and cortex presented no significant difference between blank control （CC） group and environment control （TC） group, but the expression of nNOS mRNA increased in REM sleep deprivation for 1 d and 3 d groups. The expression of nNOS mRNA was significantly increased in REM sleep deprivation for 5 d group, The expression of nNOS mRNA achieved peak in REM sleep deprivation for 7 d group （0.691 ± 0.008, P 〈 0.01）. The expression of nNOS mRNA in sleep recovery for 6, 12 and 24 h groups was still higher than that in TC group. Conclusion Deprivation of REM sleep increased the expression of nNOS mRNA in hippocampus and cortex of rats.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2005年第5期304-306,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
国家自然科学基金资助项目(30270487)
上海市科技发展基金资助项目(024119029)
关键词
睡眠剥夺
海马
聚合酶链反应
一氧化氮合酶
sleep deprivation
hippocampus
polymerase chain reaction
nitric-oxide synthase
