摘要
目的:探讨褐藻多糖硫酸酯(Fucoidan,FPS)对阿霉素肾硬化大鼠肾脏的保护作用。方法:将32只SD大鼠分成4组:假手术组(SHAM组),阿霉素肾硬化模型组(M组),褐藻多糖硫酸酯治疗组(FPS组),洛汀新治疗组(B组)。8周后观察各组大鼠24 h尿蛋白定量、血尿素氮(BUN)、肌酐(Scr)及肾脏病理改变,免疫组化观察肾脏组织纤连蛋白(FN)、Ⅳ型胶原(ColⅣ)、转化生长因子β1(TGF-β1)的表达。RT-PCR测定肾皮质转化生长因子β1(TGF-β1)和纤溶酶原激活物抑制物(PAI-1)mRNA。结果:FPS能减少尿蛋白、降低BUN和Cr,减轻基质增生和肾小球硬化,且能减少TGF-β1、FN、ColⅣ表达,下调肾皮质TGF-β1P、AI-1 mRNA表达。结论:FPS能减轻阿霉素肾硬化大鼠肾脏损害,对肾脏有保护作用。
Objective： To investigate the effects and mechanism of fucoidan on adriamycin-induced glomerulosclerosis in rats. Methods： Thirty-two Sprague Dawley rats were randomly divided into sham operation, glomerulosclerosis model, fucoidan treatment, and valsartan groups. The 24-hour urinary protein excretion, renal function were measured after 8 weeks. Renal pathology and immunohistochemistry were assayed to measure the expression of fibronectin（FN）, collagen Ⅳ （ Col Ⅳ）, tmnsformor growth factor β1 （ TGF-β1 ）. RT-PCR was used to examine the TGF-β1 mRNA and plasminogen activator inhibitor-1（PAI-1） mRNA. Result： The proteinuria, BUN, and serum creatinine declined significantly （P 〈 0.01）, and the expression of FN, Col Ⅳ, TGF-β1, TGF-β1 and PAI-1 decreased by fucoidan（ P 〈 0.01）. Conclusion： Fucoidan plays an important role on prevention and treatment for glomerulosclerosis.
出处
《山东大学学报（医学版）》
CAS
北大核心
2006年第3期291-295,共5页
Journal of Shandong University:Health Sciences
