摘要
目的：观察葛根素对血管平滑肌细胞（VSMC）增殖的影响及对c-fos和bcl-2蛋白的表达作用，旨在认识葛根素作用的分子机制。方法：建立凝血酶（T）诱导VSMC增殖的模型，以细胞计数和流式细胞仪DNA含量测定、细胞周期分析法观察T及葛根素对VSMC增殖和DNA合成的影响。T及葛根素等各处理因素作用24h后，用免疫印迹法（Western blot）检测c-fos蛋白和bcl-2蛋白表达。结果：T对VSMC有明显促增殖作用，促增殖效应在24h末达峰，且T在0．1-1．0U·L^-1有剂量依赖关系；葛根素呈剂量依赖性地抑制T诱导的细胞增殖、DNA合成以及VSMC c-fos和bcl-2蛋白的表达。结论：葛根素能抑制T诱导的VSMC增殖，这可能与其抑制c-fos和bcl-2蛋白表达有关。
Objective： To observe the role of puerarin on the proliferation of vascular smooth muscle cells（VSMC） induced by thrombin （T） and the effect of puerarin on the c-los and bcl-2 protein expression. Method： Cell number and cell cycle analysis using flow cytometry were adopted as two different indicators of effects on proliferation of VSMC. Western blot was used to indicate the changes of c-los and bcl-2 protein after 24 b of treatment of T and puerarin. Result： 1.5 × 10^- 5 - 1.5 × 10^- 3 mol· L^- 1 puerarin could significantly suppress this stimulation of VSMC proliferation and DNA synthesis induced by T. Western blot demonstrated that after 24 hour of treatment with T and puerarin, T could significantly increase c-fos and bcl-2 protein and 1.5 × 10^-5 - 1.5 ×10^-3 mol·L^-1 puerarin could significantly suppress this increase. Conclusion： puerarin can suppress the proliferation and DNA synthesis of VSMC promoted by T. This inhibitory effects of puerarin are closely related with the suppression of c-fos and bcl-2 protein.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第6期490-493,共4页
China Journal of Chinese Materia Medica
关键词
凝血酶
葛根素
血管平滑肌细胞
增殖
thrombin
puerarin
vascular smooth muscle cell
proliferation
