摘要
目的研究旋转恒定磁场对5-氟尿嘧啶(5-FU)损伤小鼠造血功能的保护作用。方法BalB/C小鼠随机分为对照组和磁场组,各组小鼠分别腹腔注射致死剂量(180 mg/kg、210 mg/kg、250 mg/kg)及亚致死剂量(150 mg/kg)5-FU,磁场组经磁场处理30 d,每天2次,每次1 h,磁场强度0.6 T。观察注射致死剂量小鼠30 d的存活率和存活期;检测注射亚致死剂量小鼠注射后第7、10、14、21、28天的外周血细胞数;测定第8、10、14天骨髓单个核细胞数(BMNC)及粒/巨噬细胞集落形成单位(CFU-GM)数;观察股骨骨髓病理切片、计数血管数并检测骨形态发生蛋白(BMPs)的表达水平。结果5-FU 180 mg/kg组,对照组和磁场组30 d的平均存活率分别为66.7%、100%,平均存活期分别为(23.3±0.9)d、(30±0)d;剂量210 mg/kg组,对照组和磁场组30 d的平均存活率分别为40%、100%,平均存活期分别为(17±1.2)d、(30±0)d;剂量250 mg/kg组,对照组和磁场组30 d的平均存活率分别为20%、70%,平均存活期分别为(11.5±1.6)d(、23.2±2.1)d(P<0.01);磁场组的外周血细胞数、BMNC数、CFU-GM数均比对照组增加(P<0.05);骨髓病理切片显示,对照组和磁场组骨髓内的血管数分别为(2.69±0.56)和(9.13±2.65)(P<0.01),磁场组的骨髓BMPs表达水平亦明显高于对照组。结论旋转恒定磁场对5-FU损伤小鼠具有明显的保护作用,能促进化疗损伤后造血细胞和造血微环境的修复。
Objective To study the hemoprotective effects of rotating and stationary magnetic field （RSMF） in mice treated with 5-Fluorouracil（5-FU）. Methods The BalB/C mice were randomly divided into control group and RSMF treated group. The mice were injected with 5 FU, the dose were 150, 180, 210, 250 mg/kg respectively. RSMF-treated group was exposed to RSMF for 1 h a time, twice a day during 30 consecutive days, and the magnetic intensity was 0.6 T. The survival rate and survival days during the 30 days were observed. 7, 10, 14, 21, 28 days after injection, the peripheral blood cells were counted. On day 8, 10 and 14, the number of bone marrow mononuclear cells （BMNC） and the forming ability of colony-forming unit-granulocyte/macrophage （CFUGM） were measured. The pathological section of femur and the expression level of bone morphogenic proteins （BMPs） in bone marrow were evaluated. Results RSMF could increase the survival rate and survival days of mice treated with 5 FU, and induce an increase in hemoglobin concentration, white blood cell count （WBC）, red blood cell count （RBC） and platelet number. Also, RSMF could increase the number of BMNC and improve the forming ability of CFU-GM on days 8 ～ 14. Furthermore, RSMF could improve the bone marrow angiogenesis and the expression level of BMPs, Conclusion RSMF have an obvious protective effect against chemotherapeutic injury, and it can accelerate the recovery of hematopoiesis and hematopoietie microenvironment in mouse bone marrow.
出处
《中国康复理论与实践》
CSCD
2006年第3期213-216,F0003,共5页
Chinese Journal of Rehabilitation Theory and Practice
基金
深圳大学科研启动基金项目(200524)
关键词
旋转恒定磁场
5-氟尿嘧啶
化学疗法损伤
造血保护
小鼠
rotating and stationary magnetic field
chemotherapeutic injury
5 Fluorouracil
Hemoprotection
mouse
