摘要
目的使用高效液相色谱-质谱联用技术,研究丹酚酸B的大鼠小肠吸收。方法分别在大鼠外翻空肠中加入丹酚酸B溶液和丹参提取液,比较两种给药方式丹酚酸B的吸收,同时观察加入P-糖蛋白抑制剂维拉帕米后对丹酚酸B吸收的影响。结果丹酚酸B在单体形式下吸收较好,维拉帕米对丹酚酸B的吸收未见显著影响。结论丹参提取液中的其他成分抑制了丹酚酸B的吸收,丹酚酸B不是P-糖蛋白的底物。
OBJEVTIVE To develop a HPLC-MS method to study the absorption of salvianolic acid B in the rats small intestine. METHODS The rat everted gut sac was incubated with salvianolic acid B solution and the decoetion of Radix Salviae miltiorrhizae, respectively,The ＇absorption of salvianolic acid B between two methods was compared. The influence on the absorption of salvianolic acid B was investigated after the addition of verapamil, a P-glycoprotein inhibitor, RESULTS The absorption of salvianolic acid B in monomer solution was higher than that in decoction, verapamil showed no effect on the absorption of salvianolic acid B. CONCLUSION Other constituents in the decoction of Radix Salviae Mihiorrhizae reduce the absorption of salvianolic acid B. Salvianolic acid B is not the substrate of P-glycoprotein.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第7期545-547,共3页
Chinese Pharmaceutical Journal
基金
国家自然科学基金重大研究计划重点项目(90209005)
国家重点基础研究发展计划(973计划)(2005CB523402)
关键词
丹酚酸B
丹参
小肠吸收
液-质联用
salvianolic acid B
Radix Salviae Miltiorrhizae
intestinal absorption
HPLC-MS
