摘要
目的热性惊厥(febrile se izure,FS)是婴幼儿时期最常见的惊厥性疾患之一,阐明其发生机制一直是该领域的重要研究课题。该课题前期的研究证明,γ-氨基丁酸B受体(-γam inobutyric ac id B receptor,GABABR)亚基和气体信号分子硫化氢(H2S)均在反复热性惊厥中发挥了重要作用。该文使用GABABR激动剂ba-c lofen,抑制剂Phac lofen,探讨GABABR对FS大鼠硫化氢/胱硫醚-β-合成酶(cystath ion ine--βsynthase,CBS)体系表达的影响。方法大鼠随机分为对照组,FS组,FS+bac lofen组,FS+phac lofen组。采用热水浴诱导大鼠FS,隔日诱导1次,共10次。采用分光光度计法测定大鼠血浆中H2S含量;用原位杂交方法观察CBS mRNA表达情况;用免疫组化方法观察CBS蛋白表达情况。结果FS+bac lofen组H2S含量较FS组升高427.45±15.91μmol/L vs362.14±19.71μmol/L,同时CBS表达也较FS组增强;而FS+phac lofen组H2S含量较FS组降低189.72±21.53μmol/L vs 362.14±19.71μmol/L,同时CBS表达也较FS组减弱。结论反复热性惊厥过程中,GABABR的改变可影响H2S/CBS体系的表达。
Objective Febrile seizure （FS） is one of the most common seizure types in children. Our previous studies have demonstrated that both γ-aminobutyric acid B receptor（GABABR） and hydrogen sulfide（ H2S） are involved in the pathogenesis of FS. This study was designed to explore the effect of GABABR on H2S / cystathionine-β-synthase （CBS） system in recurrent FS. Methods Sixty-four Spragne-Dawley rats aged 21 days were randomly assigned into four groups： Control （37℃ water bath exposure）, FS, FS ＋ baclofen （GABABR excitomotor）, and FS ＋ phaclofen （GABABR inhibitor） groups （ n=16 each）. FS was induced by warm water bath exposure （45.2℃）, once every 2 days, 10 times in total. The plasma level of H2S was detected by the spectrophotometer. The expression of CBS mRNA was examined by in situ hybridization. The expressions of CBS protein was observed by immunohistochemistry. Results The plasma level of H2S increased in the FS ＋ baclofen group （427.45±15.91μmol/L） but decreased in the FS ＋ phaclofen group （ 189.72±21.53 μmol/L） compared with that in the FS group （362.14±19.71μmol/L）. The expressions of CBS mRNA and protein were up-regulated in the FS ＋ baclofen group but were down-regulated in the FS ＋ phaclofen group compared with those in the FS group. Conclusions GABABR modulated the expression of H2S/CBS system in recurrent FS.
出处
《中国当代儿科杂志》
CAS
CSCD
2006年第2期141-143,共3页
Chinese Journal of Contemporary Pediatrics
基金
卫生部临床学科重点项目基金资助(20010912)
关键词
惊厥
发热性
γ-氨基丁酸
受体
硫化氢
胱硫醚-Β-合成酶
大鼠
Seizure, febrile
Gamma-aminobutyric acid
Receptors
Hydrogen sulfide
Cystathionine-β-synthase
Rats
