摘要
目的:抗体蛋白质芯片初步筛选常见细胞因子在卵巢癌耐药细胞株中的表达。方法:对6株卵巢癌细胞分别进行平行药敏试验及细胞因子抗体蛋白质芯片检测,同时筛选78种细胞因子的表达差异,并进行两两比较。结果:不同种类卵巢癌细胞系对于ADM的IC50值从高到低前3位依次为SKOV3,OVCAR5,OVCAR4;CBPDA为I-GROV1,SKOV3,OVCAR3;TAXOL为OVCAR4,SKOV3,IGROV1;VP16为OVCAR4,OVCAR5,OVCAR8。在对一线化疗药ADM和CBPDA耐药性较高的SKOV3细胞中GRO,IL-6,IL-8和TIMP-2均较其余5株相对敏感细胞增高,对二线化疗药TAXOL,VP16耐药性较高细胞株OVCAR4中IL-6,IL-8较之IGROV1,OVCAR3,OVCAR5升高。结论:在常见分泌型细胞因子中,GRO,IL-6,IL-8和TIMP-2升高与一线化疗药ADM和CBPDA耐药性相关,IL-6,IL-8与二线化疗药TAXOL,VP16耐药性相关,不同来源的卵巢癌细胞分泌型蛋白作用机制可能存在共同的上述几种蛋白参与的调节体系。
AIM： To identify the key factors responsible for drug resistance in different ovarian cancer cell lines using protein microarray system. METHODS： Six ovarian cancer cell lines were employed. The sensitivity of ovarian cancer cell line to common chemotherapeutic drugs was determined by using MTF assays. The expression of 78 cytokines and other factors was examined by using cytokine antibody array technology. RESUTS： Different ovarian cancer cell line responded to chemotherapeutic agents differently. The drug resistance was correlated with certain cytokine expression. Cell line SKOV3 was less sensitive to first line chemotherapeutic drug （ADM, CBPDA） and accumulated high amounts of GRO and TIMP-2 compared with other 5 cell lines. OVCAR4 cells were more resistant to second line chemotherapeutic drug （TAXOL, VP16） and had higher levers of IL- 6 and IL- 8 than IGROV1, OVCAR3 and OVCARS. CONCLUSIONS： Among the most contain excretive cytokines, increasing of GRO, IL - 6, IL- 8 and TIMP - 2 might be related to drug - resistance of ADM and CBPDA in ovarian cancer cell, while IL- 6 and IL- 8 might also be related with drug resistance of TAXOL and VP16. The different types of ovarian carcer cell might have roughly similar exceretive cytokines - induced mechanism of drug resistance.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第4期766-770,共5页
Chinese Journal of Pathophysiology
关键词
蛋白质芯片
卵巢肿瘤
细胞因子
抗药性
肿瘤
Protein microarray
Ovarian neoplasms
Cytokines
Drug resistance, neoplasm
