摘要
目的:探讨核因子kB(NF-kB)对低氧大鼠肺动脉平滑肌细胞(PAMSC)内皮素-1(endothelin-1,ET-1)表达的影响。方法:分离培养大鼠肺动脉平滑肌细胞,分别在常氧和低氧条件下培养48小时。ELISA检测培养上清中ET-1含量,RT-PCR检测ET-1 mRNA表达。在培养液中加入NF-kB抑制剂PDTC,检测PASMCs ET-1表达的变化。Western blotting检测PASMCs IkB表达变化。结果:低氧培养能够诱导PASMCs表达ET-1。NF-kB抑制剂能够减少由于低氧引起的ET-1释放,IkB在低氧情况下表达明显减少。结论:ET-1低氧情况下在PAMCS表达明显增加,可能参与低氧所引起的肺动脉的病理过程。低氧所引起的ET-1表达增加可能通过NF-kB信号通路。
Objective： To study the effects of the nuclear factor kappa B （NF- kB ） in the endothelin- 1（ET- 1） expression in the PASMCs of rats under hypoxia. Methods： 28 male Wistar rats wero selected and their PASMCs were isolated and incubated at normal situation or under hypoxia for 48 hours. The ET - 1 level in the supematant was detected by ELISA. RT - PCR was used to investigate the mRNA expression of ET-1 in the PASMCs. The IkB, which is the most prominent indicator of activation of NF - kB, was detected by Western blotting in the PASMCs. The inhibitor of NF- kB（10uM）, PDTC, was added to the culture to investigate the effect of it on the expression of ET-1. Results： The ET- 1 expression can be induced significantly in the hypoxia situation. The inhibitor of NF - kB can decrease the expression of ET - 1 induced by hypoxia. The IkB expression decreased significantly under hypoxda. Conclusion： The expression of ET- 1 increased obviously in the PASMCs under hypoxia, which may participate in the pathological events of the PASMCs in the hypoxia situation and contribute to the activation of NF- kB signal pathway. So inhibiting NF- kB activation could be a new therapy for pulmonary artery hypertension induced by hypoxia.
出处
《现代生物医学进展》
CAS
2006年第2期39-41,共3页
Progress in Modern Biomedicine
关键词
低氧
肺动脉高压
内皮素-1
核因子-KB
Hypoxia
Pulmonary artery hypertension
Endothelin - 1
Nuclear- factor kappa B
