摘要
目的:研究细胞凋亡及凋亡相关蛋白bcl-2在肺癌组织中的表达水平,及其对预后的影响。方法:应用DNA缺口末端标记技术和免疫组化方法检测116例肺癌患者组织中细胞凋亡和bcl-2蛋白表达水平。结果:116例肺癌患者中,细胞凋亡数量达到或超过0·1%的病例占48·3%(56/116),bcl-2蛋白阳性表达的病例占51·7%(60/116);单因素分析显示,肺癌患者预后的影响因素有凋亡(χ2=8·31,P=0·0039)、bcl-2(χ2=4·41,P=0·0357)、淋巴结转移(χ2=19·34,P=0·0001)和TNM分期(χ2=18·54,P=0·0001);COX模型多因素分析显示,淋巴结转移(χ2=19·28,P=0·0010)和bcl-2蛋白阳性表达(χ2=5·80,P=0·0160)是肺癌患者的预后不良因素。结论:凋亡及凋亡相关蛋白bcl-2影响肺癌患者的生物学行为及预后。
OBJECTIVE：To investigate prognostic significance of the expressions of apoptosis and its correlating protein bcl-2 in lung cancer. METHODS： The expression of apoptosis and the positive expression of bcl-2 protein were observed in 116 cases of lung cancer by using DNA nick end labeling （TUNEL） technique and immunohistochemical staining. RESULTS： In the specimens of 116 lung cancer,the expressions of cell apoptosis equal to or more than 0.1% were 48. 28%（56/116）, and the positive rates of bcl-2 protein expressions was 51.72% （60/116）. Single factor analysis showed that apoptosis （x^2 = 8. 31, P = 0. 003 9）, bcl-2 （x^2 = 4.41, P=0.035 7）, the metastasis of lymph node （x^2=19.34,P= 0.000 1） and TNM stage （x^2=18.54,P=0.000 1） affected the prognosis of lung cancer patients. COX model showed that the metastasis of lymph node （x^2= 19.28,P=0. 001 0） and the positive rate of bcl-2 protein （x^2 =5.80,P=0. 016 0） were poor factors to the prognosis of lung cancer patients. CONCLUSIONS： Apoptosis and the correlated protein bcl-2 are the factors that affect the biology behavior and prognosis of lung cancer patients.
出处
《中华肿瘤防治杂志》
CAS
2006年第4期290-293,共4页
Chinese Journal of Cancer Prevention and Treatment
