摘要
目的探讨AE1/AE3免疫组化标记检测胃癌根治标本中淋巴结内微小转移癌及其临床意义。方法对64例胃癌病人根治切除后阴性的淋巴结及胃周软组织的石蜡包埋组织用S-P法进行AE1/AE3染色标记。结果64例118枚淋巴结中,根据HE染色阴性再经AE1/AE3标记发现,8例患者13枚淋巴结有微转移灶,病例总阳性率12.50%(8/64),淋巴结总阳性率11.02%(13/118)。微转移与浸润深度有关,深层组织的微转移阳性率高于浅层(P<0.05),弥漫型胃癌微转移率(62.50%)明显高于肠型胃癌(5.36%),两者之间具有显著差异性。结论AE1/AE3染色标记检测微转移淋巴结可能对准确地确定临床分期、诊疗及判断预后有积极临床意义。
Objective To study the lymph node micrometastasis of gastric cancer and its clinical significance. Methods Immunohistochemistry method of S-P was used to examine AE1/AE3 in 118 lymph nodes of 64 primary gastric cancer patients. Results Micrometastases were found in 13 lymph nodes （11.02%） of 8 patients （12.5%）. The incidence of micrometastasis was correlated with the depth of invasion, the incidence of micrometastasis in deeply invaded tissues was more than that in superficially invaded tissues （P 〈 0.05）. The incidence of micrometastasis in wide spread gastric cancer （62.50%） was much higher than that in gastric cancer of colon type （5.36%） and the difference between them was significant （P 〈 0.05）. Conclusion The micrometastases exist in the visibly normal lymph nodes in the gastric cancer patients and the micrometastasis detection by AE1/AE3 in negative lymph nodes of gastric cancer patients is recommended to apply to precisely determine the tumor stage, and direct cancer therapy and predict prognosis.
出处
《现代消化及介入诊疗》
2006年第1期3-5,共3页
Modern Interventional Diagnosis and Treatment in Gastroenterology
