摘要
目的:比较顺铂或奥沙力铂联合热疗对人未分化胃腺癌细胞系BGC823增殖的抑制作用。方法:使用MTT法检测细胞增殖,确定顺铂和奥沙力铂对BGC823抑制的工作浓度,并以该浓度进行化疗或与热疗联合应用,根据Veleriote法判断热、化疗联合应用后24h或48h的作用效果,并将顺铂和奥沙力铂联合热疗的作用进行比较。结果:以20%～30%抑制率的药物浓度作为试验的工作浓度,确定顺铂和奥沙力铂的工作浓度分别为1μg/ml和5μg/ml。单纯43℃热疗1h后,在24h时和48h时对BGC823的抑制作用无统计学意义(P>0.05);应用顺铂1μg/ml和奥沙力铂5μg/ml后24h对BGC823无显著的抑制作用(P>0.05),48h时呈现显著的抑制作用(P<0.01)。应用两药联合43℃热疗1h,在24h和48h对BGC823均有显著的抑制作用(P<0.05),随着时间的延长,对BGC823的抑制作用增强(P<0.05)。单药化疗在24h或48h时,两药的抑制作用无统计学差异(P>0.05),顺铂联合热疗对BGC823的抑制作用在24h和48h时均比奥沙力铂联合热疗的抑制作用强(P<0.05)。根据Veleriote法判断:顺铂1μg/ml联合热疗在24h和48h时均呈明显的协同作用;奥沙力铂5μg/ml联合热疗在24h时产生次加作用,在48h产生协同作用。结论:顺铂或奥沙力铂联合热疗在24h和48h时呈现次加或协同作用,两种药物相比,顺铂比奥沙力铂作用更强。
To compare the anticancer effect of cisplatin or oxaliplatin combined with hyperthermia against human undifferentiated gastric adenocarcinoma cell line BGC-823. Methods ： The working concentrations of cisplatin and oxaliplatin against BGC-823 were determined by MTT assay that has been developed for quantitative evaluation of the proliferation of cells. Then the chemotherapy or hyperthermia, singly or concurrently, were used and the cell survival rates were obtained. The anticancer effect was evaluated and compared by Veleriote method at 24 h or 48 h. Results ： The working concentrations were defined as the concentration causing 20% ～30% inhibition rate. They turned out to be 1μg/ml and 5 μg/ml of cisplatin and oxaliplatin, respectively. Exposing to 43 ℃ hyperthermia for 1 hour did not show obvious inhibition to BGC-823 at 24 h or 48 h （P 〉 0.05 ）. The chemotherapies with cispla- tin 1 μg/ml or oxaliplatin 5μg/ml did not exhibit obvious inhibition to BGC-823 at 24 h （ P 〉 0.05 ）, but they did at 48 h（ P 〈 0.01 ）. The combination of cisplatin or oxaliplatin with 43℃ hyperthermia for 1 hour showed obvious inhibition to BGC-823 at 24 h and 48 h. （P 〈 0.05 ）. And the effect of chemohyperthermia was amplified as the time prolonged （P 〈 0.05 ）. There was no difference between the two drugs at 24 h and 48 h when used singly. The inhibitory effect of cisplatin was much higher than that of the oxaliplatin when used with hyperthermia. Judged with the Veleriote method at 24 h and 48 h, there was obvious synergism of cisplatin 1 ℃g/ml combined with hyperthermia. Oxaliplatin combined with hyperthermia exhibited subaddictive effect at 24 h and synergism at 48 h. Conclusion ： The combination of cisplatin or oxaliplatin and 43℃ hyperthermia for 1 hour showed subaddictive effect or synergism to the inhibition of human undifferentiated gastric adenocarcinoma cell line BGC-823, and cisplatin is much better than oxaliplatin when combined with hyperthermia.
出处
《医学研究生学报》
CAS
2006年第4期310-314,共5页
Journal of Medical Postgraduates
基金
江苏省卫生厅重点基金资助项目(批准号:H200349)
关键词
胃癌细胞系
热疗
热化疗
顺铂
奥沙力铂
Gastric cancer cell line
Hyperthermia
Chemohyperthermia
Cisplatin
Oxaliplatin
