摘要
为了探明转CP4-EPSPS(5-烯醇丙酮酸莽草酸-3-磷酸合成酶)基因是否会对大豆种子及幼苗的抗逆性产生影响,进而为转基因耐草甘膦大豆的环境安全评价提供科学依据,通过室内试验研究了温度、水分和盐度对转基因耐草甘膦大豆ARG、受体大豆n-ARG及我国传统栽培大豆苏豆3号种子萌发与幼苗生长的影响。结果表明:在种子萌发阶段,ARG对高温胁迫和水分胁迫的抗性显著低于n-ARG和苏豆3号;ARG对盐分胁迫的抗性与n-ARG没有显著差异,但抗性明显弱于苏豆3号。幼苗生长阶段,在温度胁迫(高温40℃,低温15℃)和水分胁迫(渍水和干旱)条件下,ARG在株高增长量和生物量方面都没有表现出优势,相反,在某些方面反而抗性更弱。例如,在轻度干旱胁迫(相对含水量55%)条件下,ARG地上部干重明显小于n-ARG。ARG对盐分胁迫的抗性也弱于n-ARG及我国传统栽培大豆苏豆3号。总之,与非转基因受体大豆n-ARG和我国传统栽培大豆苏豆3号相比,转基因耐草甘膦大豆ARG在温度、水分和盐度逆境条件下的竞争性和入侵性不强。
To ascertain whether transgenic engineering will affect resistance of seeds and seedlings to stresses,and consequently provide first-hand scientific information for environmental safety assessment of transgenic soybean, comparative studies were conducted in laboratory and greenhouse on transgenic soybean ARG,it＇s counterpart n-ARG and Su 3 a traditional soybean cultivar in China. Results show at the germination stage, ARG was much weaker than n-ARG and Su 3 in resistance to high temperature and water stress, and similar to n-ARG but weaker than Su 3 in resistance to salt stress. At the seedling stage, given temperature stress（ high-40℃ ,low- 15℃ ）and water stress（ flood and drought）, ARG showed no advantage in height and biomass, and on the contrary,some disadvantages in certain conditions. For example, under a slight drought（ relative water content 55% ）, ARG was obviously less than n-ARG in dry weight of the overground part, and also weaker than n-ARG and Su 3 in salt tolerance. The research shows the herbicide-tolerant transgenic soybean ARG is rather weak in competitiveness and invasiveness under stress conditions.
出处
《生态与农村环境学报》
CAS
CSCD
北大核心
2006年第2期26-30,共5页
Journal of Ecology and Rural Environment
基金
科技部重大转基因植物研究专项(J00-C-004)
关键词
温度
水分
盐度
转基因耐草甘膦大豆
发芽率
幼苗
temperature
moisture
salinity
transgenic soybean
tolerant to glyphosate
seedling
