摘要
目的研究不同药物雾化吸入对重型颅脑损伤大鼠肺泡巨噬细胞吞噬功能的影响。方法采用大鼠颅脑局部气压冲击伤模型,将58只雄性SD大鼠随机分为正常组、生理盐水组、庆大霉素组及γ-干扰素组。正常组4只动物不作任何处理,其余3组每组18只动物,均于致伤后第1天开始分别给予生理盐水、生理盐水+庆大霉素、生理盐水+γ-干扰素雾化吸入,2次/d,观察1、3、7 d处死动物,用支气管肺泡灌洗获取标本,用白色念珠菌法观察肺泡巨噬细胞的吞噬率。结果γ-干扰素组3 d和7 d肺泡巨噬细胞的吞噬率(0.742±0.073、0.778±0.033)高于生理盐水组(0.312±0.040、0.560±0.075)和庆大霉素组(0.388±0.074、0.415±0.089),P<0.001。结论γ-干扰素雾化吸入可提高重型颅脑损伤大鼠肺泡巨噬细胞吞噬率,庆大霉素吸入7 d时可使肺泡巨噬细胞吞噬率下降。
Objective To study the influence of different inhaling formula via aerosol on the phagoeyting function of alveolarmacrophage in the lungs of rats with severe head injury. Methods The model of severe head injury （SHI） was established by localimpact injury. Fifty-eight male SD rats were divided randomly into group N（n=4）, group C （n=lS）, group A （n=18）, group B （n=18）.Group C was inhaled normal saline via aerosol; Group A was inhaled normal saline plus gentamycin via aerosol, Group B inhaled nor-mal saline plus γ-IFN via aerosol after injury. The phagocyting function of alveolar maerophage was investigated by Oidium albicans.Results The phagocyting percentage of group B （0.742±0.073, 0.778±0.033） were markedly higher than that of group C （0.312±0.040, 0.560i-0.075） and group A （0.388±0.074, 0.415±0.089） on Day 3 and Day 7（P〈0.01）. Conclusion Administration of γ-IFNvia aerosol can obviously enhance the phagocyting percentage of alveolar macrophage in the lungs of rats with severe head injury,while gentamycin can reduce the phagocyting percentage on day 7.
出处
《护理学报》
2006年第4期11-13,共3页
Journal of Nursing（China）
