摘要
作者自网箱养殖的卵圆鲳(Trachinotusblochii)分离了一株刺激隐核虫(Cryptocaryonirritans),再经人工感染的方法收集各期虫体,制成电镜样品,对虫体进行超微结构研究。研究表明:刺激隐核虫的毛基体具有典型的9套三联微管系统。幼虫体部单毛基单元有如下特征:单毛基单元结构,具有稍分支的纤毛后微管束(Postciliarymicrotubularribbon),由3根微管组成,起源于第3三联微管,向后延伸至细胞表面。滋养体体部单毛基单元特征是具有分支的纤毛后微管束,由3-6根微管组成,起源于第9三联微管。毛基体的第4-7组三联微管处发出一条长而无分支重叠的动纤丝(KD),KD向右前方伸延到质膜脊的顶端。在横微管(T)左侧的第3三联微管处有一个横行支持物即横带,由3根微管组成,与来源于第3-4三联微管的横纤维(Tf)相连。刺激隐核虫的体部毛基单元具有一个独特特征:即具有体部线带(N),是一束平行排列的微管,它们起源于邻接横微管和横纤维的致密板,滋养体的体部线带相互重叠交错排列,以此支撑细胞的横轴。滋养体期还有厚的表质层(Epiplasmiclayer)。体部双毛基单元即为邻近口区的双毛基单元,每一排动基列的前几个毛基单元都是双毛基单元,极似口部毛基单元,口部双毛基单元的后面有3-4个体部双毛基单元。幼虫和滋养体的体部双毛基单元均具有2个毛基体,其结构模式相似。体部毛基单元的2个毛基体各长出1根纤毛,毛基体与一个旁体囊(PS)相连,其前面有动纤丝。文中分析了刺激隐核虫在形态上与淡水小瓜虫以及相关种类的毛基单元和皮层结构差异,认为刺激隐核虫的分类更适合归于前口类(Prostomatea),而不是膜口类(Hymenostomatida)[动物学报52(2):396-405,2006]。
The morphology of an isolate of parasitic ciliate Cryptocaryon irritans obtained from cage-cultured snubnose pompano Trachinotus blochii from Huidong, Guangdong province in China, was examined by transmission electron microscopy （TEM）. The strain was maintained in experimentally infected snubnose pompano from which the sample used for ultrastructural observations was collected. The somatic kinetosomes of Cryptocaryon showed the classic morphology of nine triplets of microtubules. The theronts＇ somatic monokinetid structure is characterized as follows： A ciliated monokinetid with a slightly divergent postciliary microtubular ribbon consisiting of three microtubules, originates at triplet number 9 and extends posteriorly towards the cell surface. The trophont＇ s somatic monokinetid is characterized as follow： A ciliated monokinetid with a slightly divergent postciliary microtubular ribbon consisting of 3 to 6 microtubules, originates at triplet number 9. An anteriorly extending, long but non-overlapping kinetodesmal fibril adheres to the anterior face of the kinetosome at triplets 4 - 7. It extends into the apexes of the pellicular crest. A transverse support is present adjacent to triplet 3 at the left side of the transverse ribbon, consisting of three microtubules and associated with the transverse fibril which originates between triplets 3 - 4. A novel feature of the somatic monokinetids of Cryptocaryon trophonts is the presence of somatic nematodesmata which are bundles of parallel microtubules. They Originate from a dense plate close to the transverse microtubules and transverse fibril. In the trophonts, the somatic nematodesmata overlap with each other to support the transverse axis of the cell. A thickened epiplasmic layer is found in the trophont stage. The somatic dikinetids are dikinetids close to the oral area. Several of the anteriormost somatic dikinetids in each kinety appear as oral dikinetids. There are at least 3 - 4 rows of somatic dikinetids posterior to the oral dikinetids. The kinetid pattern of the somatic dikinetids is very similar in both the theront and trophont of Cryptocaryon. Both kinetosomes of the somatic dikinetids are ciliated and are associated with a single parosomal sac which is anterior to the kinetodesmal fiber. The present study suggests that the taxonomic affinities of C. irritans should lie with prostome ciliates by analysis of its different morphological characteristics of kinetids and cortex from I. multifiliis and related species .
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2006年第2期396-405,共10页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金(No.30440052)
广东省自然科学基金团队项目(No.20023002)资助~~
关键词
刺激隐核虫
超微结构
卵圆鲳鲹
皮层
Ciliate, Cryptocaryon irritans, Ultrastructure, Trachinotus blochii, Cortex
