摘要
目的探讨周期性牵张对膀胱逼尿肌细胞表达连接蛋白43(Connexin43,Cx43)的诱导作用。方法运用牵张膀胱逼尿肌细胞体外模型,给予逼尿肌细胞周期性20%拉伸度的牵张,持续6 h;采用W estern b lotting、RT-PCR在蛋白和mRNA水平测定逼尿肌细胞Cx43表达。结果正常对照组Cx43表达量为(1 1.377 5±0.425 1),Cx43mRNA相对表达量为(0.423 3±0.187 5);而给予20%牵张刺激6 h逼尿肌细胞Cx43表达量增加至(14.015 0±0.526 2),Cx43mRNA相对表达量增加到(0.935 0±0.020 67),二者均较对照组有显著增加(P<0.01)。结论周期性牵张可显著诱导增加逼尿肌细胞Cx43在蛋白和基因水平的表达。
Objective To investigate the connexin43 overexpression of detrusor smooth muscle cells （DSMCs） by cyclic stretch. Methods The DSMCs growing on collagen-coated silicone membrane were subjected to cyclic stretch-relaxation. The Cx43 and Cx43 mRNA expression in DSMCs were detected with Western blotting and RT-PCR. Results The Cx43 and Cx43 mRNA expression of control DSMCs were respectively （11. 377 5 ±0.425 1 ） and （0.423 ± 3 0. 187 5 ）, and that of DSMCs subjected to cyclic stretch-relaxation were （14.015 0 ± 0.526 2） and （0.935 0 ± 0.020 67 ）, with significant difference between the two groups （P 〈0.01 ）. Conclusion The connexin43 overexpression in DSMCs could be induced by cyclic stretch.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第9期967-969,共3页
Journal of Third Military Medical University
关键词
周期性牵张
逼尿肌
CX43
cyclic stretch
detrusor muscle
connexin43
