摘要
利用溶剂浇铸/颗粒沥滤技术制备出壳聚糖和硫酸软骨素共混膜,研究了共混膜的透光性、表面结构、红外图谱、X射线衍射图谱以及生物相容性和生物降解性.结果表明,共混膜透明度高,结构均匀,孔隙大小合适,壳聚糖分子与硫酸软骨素达到分子水平分散,且分子间存在较强的相互作用,共混膜具有良好的生物相容性和生物降解性.以共混膜为载体培养兔角膜内皮细胞,结果表明,共混膜非常适合角膜细胞贴附生长,10 d内长成良好单层细胞.将载有角膜内皮细胞的膜片植入内皮缺损兔眼中,在56 d内术眼基本保持透明,之后角膜水肿,逐渐模糊并出现新生血管,在230 d时术眼新生血管基本消失,角膜恢复透明.
Chitosan/chondroitin sulfate blend film was prepared by solution casting/particulate leaching technique and characterized by transparence, SEM, FTIR and X-ray diffraction. Its biocompatibilities and biodegradability in vitro and in vivo were also evaluated. The results indicate that the blend film had an excellent transparence, uniform structure and appropriate pore size. Chiiosan and chondroitin sulfate can disperse to molecules level and strong interaction existed between molecules. The blend film had favorable biocompatibility and biodegradability. The attachment and growth of rabbit corneal endothelial cells on the blend film was studied by cell culture. The attached cells were able to proliferate and grow near confluent. The blend film showed to be a good carrier for attachment and growth of corneal endothelial cell. Corneal endothelial cellloaded blend film was transplanted into rabbit eyes with endothelium scraped. The eyes remained transparent after surgery for 56 d, then the cornea became edema and many new blood vessels appeared. But the cornea regains transparence and new vascular disappear after surgery for 230 d.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第5期880-884,共5页
Chemical Journal of Chinese Universities
基金
国家'八六三'计划基金(批准号:2003AA625050)
国家自然科学基金(批准号:30070220)资助
关键词
壳聚糖
硫酸软骨素
共混膜
角膜内皮细胞载体
移植
Chitosan
Chondroitin sulfate
Blend film
Corneal endothelial cell carrier
Transplantation
