摘要
研究了半滑舌鳎仔、稚、幼鱼期蛋白酶和淀粉酶活力的变化及其在成鱼肝脏、前肠、中肠和后肠中的分布情况。结果表明,半滑舌鳎受精卵时期就有了一定的淀粉酶和蛋白酶活力。淀粉酶活力在30日龄和60日龄最高,2日龄最低;蛋白酶活力在8日龄最高,14日龄仔鱼、30日龄稚鱼和60日龄幼鱼略低,2日龄最小。成鱼体内淀粉酶的比活力由高到低的顺序为:肝脏>后肠>前肠>中肠,肝脏与各肠段内淀粉酶比活力差异极为显著(P<0.01);蛋白酶比活力由高到低顺序为:肝脏>后肠>中肠>前肠。肝脏内的两种酶比活力都很高。
Protease and amylase distribution in adult fish and their activity variation in larval and juvenile of Cynoglossus semilaevis Günther were studied. As observed, the amylase and protease activities could be detected in oosperm. During the larval and juvenile phase, the activities of amylase presented highest in 30-days-old and 60-days-old juveniles, lowest in 2-days-old larvae. The highest activities of protease occurred in 8-days-old larvae, relatively low in 14- days-old larvae, 30-days-old and 60-days-old juveniles. Like the amylase activity, the lowest appeared in 2-days-old larvae either. In adult, the amylase activity was different in the organs of alimentary canal： liver 〉 hindgut 〉 foregut 〉 midgut. The amylase activity in liver was significantly different from that in intestine （P 〈 0.01）. Protease activity in alimentary canal presented a gradient of liver 〉 hindgut 〉 midgut 〉 foregut. The highest activities of both amylase and protease were detected in liver.
出处
《海洋水产研究》
CSCD
北大核心
2006年第2期43-48,共6页
Marine Fisheries Research
基金
国家863高技术研究发展计划项目(2002AA603011和2004AA603320)
青岛市自然科学基金项目(052JC59)
农业部水生动物遗传育种和养殖生物学重点开放实验室经费共同资助
关键词
半滑舌鳎
仔稚幼鱼
淀粉酶
蛋白酶
比活力
Cynoglossus semilaevis Günther Larval and juvenile fish Amylase Protease Activity
