摘要
建立了8株分泌狂犬病毒单克隆抗体(McAb)的杂交瘤细胞株。用间接免疫荧光法法(IFA)和细胞酶免疫法(CEIA)测定了这8株McAb腹水的效价,其中4株CEIA效价为10~6.CEIA的敏感性比IFA高1000倍。动物中和试验证实,6株McAb具有中和活性,中和指数高者大于3 000;另2株为非中和抗体。用非酶标记和酶标记中和抗体做相互竞争抑制试验,6株中和McAb可分为3组,分别针对3个不同的抗原位点。最后用SDS-聚丙烯酰胺凝胶电泳和Westernblot法分析狂犬病毒中和抗原,显示2条带,分子量分别为61kD和110kD。所用5株酶标中和Mc-Ab均与其有特异性反应,显示两条染色带。
We have obtained eight hybridoma cell lines which produced McAbs against rabies virus ( RV), named 9E6, 2F6, 2ElO, 2E9, 4F4,4E4, 8D6 and 9E2. Six of these can secrete neutralizing ( Neu. ) McAbs. We analysed the antigen epitopes of rabies virus with Xeu. McAbs. Firstly, we examined the six Neu. McAbs by the competitive inhibition test. The results showed that six Xeu. McAbs can be classified into three different groups based on their epitopes specificity. Secondly, we examined the SDS-PAGE and Western blot patterns of RY proteins with Neu. McAbs. The results proved that the six Neu. McAbs were all specific for the RV glycoprotein. Therefore we suggest that there are at least three different epitopes in the gly-coprotein of RV.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第2期117-121,共5页
Chinese Journal of Virology
