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DNA微点阵分析丙型肝炎病毒非结构蛋白NS4B对信号传导相关基因表达的影响 被引量：1

The effect of hepatitis C virus non-structural protein NS4B on signal transduction-associated gene expression using DNA microarray
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摘要 目的采用DNA微点阵(DNAmicroarray)技术检测致癌相关基因的表达水平,研究丙型肝炎病毒(hepatitisCvirus,HCV)非结构蛋白NS4B在HCV致癌中的作用。方法通过建立稳定表达NS4B的细胞系,用微点阵技术研究NS4B对细胞基因表达的影响,实时定量RT-PCR分析微点阵结果中的两个上调基因(DKK1,FYN)和两个下调基因(AKR1C1,v-fos)的表达,对细胞中AKR1C1的活性进行酶学分析。结果在2308个信号途径相关的基因中,34个基因表达上调,56个基因表达下调。在表达有明显差异的基因中,大部分原癌基因表达上调,而大部分抑癌基因的表达下调;一些基因与细胞压力有关。实时定量RT-PCR和AKR1C1的酶学分析进一步证实了DNA微点阵的结果。结论HCV-NS4B在HCV的致癌过程中起一定作用。 Objective The aim was to study the role of NS4B in the carcinogenesis caused by hepatitis C virus（HCV） via detecting the carcinogenesis-associated gene expression using DNA microarray. Methods After stable expressing NS4B cell line established, the influence of NS4B on cellular gene expression profiling was studied using DNA microarray; the expression of two up-regulated and two down-regulated genes from the result of DNA microarray was analyzed via real-time quantitative RT-PCR; the aldo-keto reductase family 1, member C1 （AKR1C1） enzyme activity was analyzed. Results Of the 2 308 genes we examined, 34 were up-regulated and 56 were down-regulated. Oneogenes＇ expression was mainly up-regulated, whereas tumor suppressors＇ expression was mainly clown-regulated ; Some involved in cellular stress in these significant different expression genes. The results of DNA microarray were further verified via the analysis of real-time quantitative RT-PCR and AKR1C1 activity. Conclusion The results showed that NS4B might play some roles in the carcinogenesis of HCV.
作者 吕琪 郑义 宋林立 李忠新 李瑜珍 周小梅
机构地区 广州中医药大学附属深圳中医院检验科
出处 《中国病原生物学杂志》 CSCD 2006年第2期90-94,共5页 Journal of Pathogen Biology
关键词 丙型肝炎病毒 DNA微点阵 非结构蛋白NS4B Hepatitis C virus （HCV） DNA microarray non-structural protein NS4B
分类号 R373.21 [医药卫生—病原生物学]
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参考文献18

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共引文献11

同被引文献23

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中国病原生物学杂志
2006年 第2期

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