摘要
目的通过扩增Ha7.7,获得包含该EST的基因全长,以便研究该基因的生物学功能。方法以Ha7.7-GSP1和Ha7.7-GSP2和Ha7.7-GSP3为引物,RACE法扩增亚洲璃眼蜱雌成蜱唾液腺总RNA,获得基因的5′-端。根据Ha7.7和5′-端序列设计全长引物(Ha7.7-FLP+和Ha7.7-FLP-),扩增cDNA第1链获,得全长cDNA。结果分析表明,基因大小为449bp,由吸血诱导亚洲璃眼蜱成蜱唾液腺表达。结论与数据库资料比对显示,GP15为新纪录(Genbank登录号DQ020265)。
Objective To generate a full-length cDNA containing EST by amplification Ha7. 7. Mehtods The 5＇- RACE system was used to amply the total RNA from Hyalornrna asiaticurn female tick＇s salivary gland with Gene Special Primers, Ha7.7-GSPI, Ha7.7-GSPz and Ha7.7-GSP3 o Pairs of full-length primers for GP15, Ha7.7-FLP＋ and Ha7.7- FLP- were designed according to the sequence of Ha7.7 and the 5＇ -end, Full-length cDNA of GP15 was subcloned by amplification of the first strand cDNAs. Results The analysis demonstrates that this gene is 449 bp, and expresses only in the salivary glands of the fed adult H. asiaticum tick. Conclusion The comparison to EMBL/GenBank databases indicates that GP15 is a novel gene（Genbank accession：DQ020265）.
出处
《中国病原生物学杂志》
CSCD
2006年第2期113-116,共4页
Journal of Pathogen Biology
基金
科技部基础研究重大项目前期研究专项(No.2001CCA00900)。
