摘要
目的:通过建立脾虚证大鼠模型,从药代动力学方面运用放射性示踪技术动态观察脾虚证大鼠以及运用补脾法后对铁吸收和/或转运代谢过程的影响的实验,研究脾主运化/脾失健运与铁代谢的关系,探讨脾主运化生物学意义,揭示脾主运化、脾为后天之本等重要中医理论的科学内涵,并为脾的功能和/或脾虚证本质研究提供实验依据。方法:雄性SD大鼠40只,随机分为正常组、正常补脾组、脾虚组、脾虚补脾组4组,按运动模型规范化的要求造模后,4组一次性灌服59FeCl3水悬液0.2ml/220g/只,同时依照人和鼠体重换算,正常补脾组、脾虚补脾组两组即时灌服1:1四君子汤药液1.5ml±,其它两组用1.5ml±生理盐水灌胃,日1次。分别在0.5h、1h、1.5h、3h、6h、12h、24h大鼠尾部取血0.2ml,用γ放射免疫计数器测量其放射性59Fe计数,观测放射性铁经大鼠胃肠道进入全身循环系统的药代动力学变化。结果:实验研究4组对59FeCl3经大鼠胃、大、小肠消化吸收进入全身循环系统的药代动力学变化,主要比较在脾虚状态下和在补脾状态下对59FeCl3的吸收存在明显差别,补脾法促进了大鼠对59FeCl3的吸收。结论:脾虚证大鼠降低了59FeCl3的生物利用度,不能有效促进精微物质吸收,补脾法提高了大鼠铁的生物利用度,促进了59FeCl3的吸收。显示脾虚降低了大鼠铁的生物利用度,或是降低了生物利用程度,或是降低了生物利用速度,这和脾失健运,不能有效促进精微物质吸收或转运密切相关;而补脾法在很大程度上改善了这种状态,通过调理脾而调节机体物质转运系统的功能,提高了大鼠铁的生物利用度,促进了铁的吸收。
Objective:On basement of model of rat' deficient spleen syndrome,these are the dynamic observation studies of?^(59) Fe's obsorbed metabolism and /or transported metabolism course of the rat of deficient spleen syndrome and replenishing spleen method by the application of radioactivity label technology in pharmacokinetics.The experiment studies the relation of the spleen being the transferring owner or the spleen losing the transferring owner with Fe's metabolism,researches it's biology meaning of spleen being the owner of transmission,researches the science intension of important traditional Chinese medicine theory of spleen being the owner of transmission and the spleen serving as basement of the day after tomorrow,and provides the experiment basis of spleen merit ability and/or essece of deficient spleen syndrome.Methods:40 male SD rats were randomly divided into 4 groups:normal group,normal replenishing spleen group,deficient spleen group,deficient spleen-replenishing spleen group.According to the requirement of the animal model standardizes most of rats were made the pattern.4 goups were once iffigated shui xuanye of?^(59) FeCl_3 0.2ml/220g/a rat.At the same time,according to weight of a person and a rat,2 groups of normal replenishing spleen group and deficient spleen-replenishing spleen group were given 1.5m±medicinal ingredients liquid of 1:1 four-man decoction,the others were given 1.5m±physiological saline,once a day.Respectively at 0.5h,1h,1.5h,3h,6h,12h and 24h,blood 0.2ml of all rats were taken from rat's tail,measured its radioactivity?^(59) Fe with γ-radiation immunity counter,to observe changes of pharmacokinetics of?^(59) Fe passing through rat's stomach and bowel way into the whole body blood-system.Results:The experiment exists the clear difference for changes of radioactivity?^(59) Fe pharmacokinetics in the states of deficient spleen and replenishing spleen to absorb?^(59) FeCl_3 from rat's stomach and bowel way into the whole body blood-system.Replenishing spleen method have promoted the absorb to?^(59) FeCl_3.Conclusions:The rat of deficient spleen syndrome has reduced the bioavailability of?^(59) FeCl_3 and made it the lower lever to absorb of?^(59) FeCl_3.On the contrary,Replenishing spleen method has promoted to absorb?^(59)(FeCl_3).from rat's stomach and bowel way into the whole body blood-system.
出处
《中医药信息》
2006年第3期62-64,共3页
Information on Traditional Chinese Medicine
关键词
脾虚证
补脾法
59^FeCl3代谢
药代动力学
实验研究
Deficient Spleen Syndrome
Replenishing Spleen Method
^(59) Fe's Metabolism
Pharmacokinetics
Experiment Research
