摘要
目的了解北京地区2003年流行的麻疹病毒的优势基因型。方法采集2003年北京地区暴发和散发麻疹临床病例咽喉拭子，使用WHO推荐的Vero／SLAM细胞进行麻疹病毒分离。用逆转录-聚合酶链反应（RT-PCR）扩增麻疹病毒分离株的核蛋白（N）基因碳末端450个核苷酸。对扩增产物的核苷酸序列进行测定和分析，并与麻疹病毒基因库中参考株进行比较，参考株包括H1a基因型9株，H1b基因型2株，H1c基因型7株，H2基因型1株，A基因型3株。结果从北京的7个行政区县的麻疹患者的咽喉拭子中共分离到8株麻疹病毒，经对分离株的核蛋白（N）碳末端450个核苷酸进行序列测定和分析，并与国内基因库中的22个参考株进行基因亲缘性关系比较，该8株麻疹病毒均为H1a基因型。该8株病毒与H1a参考株Chin9322、H1b参考株Chin9475和H1c参考株Chin9427的平均遗传距离分别为0．004—0．011，0．026—0．031和0．015—0．022；而与H1a参考株中的Anhui01-1，Anhui02-2的平均遗传距离最近，为0．000—0．009。结论2003年北京地区流行的麻疹病毒的优势基因型为H1a基因型，与北京往年的本土流行株存在明显核苷酸差异，H基因型的H1b、H1c以及H2基因型在北京地区可能已经消失。
Objective To investigate genotype of wild-type measles viruses circulated in Beijiag in 2003. Methods Throat swabs specimens were collected from patients seen during an outbreak of measles and from clinically suspected sporadic measles patients in 2003. Vero/SLAM cell lines recommended by WHO were used to isolate measles virus. Four hundreds and fifty nucleotides of COOH-terminal of nucleoprotein （N） genes were amplified by using PR-PCR. The amplified products were sequenced and the sequences were compared with references viruses from GeneBank. Results Eight strains of measles viruses were isolated from throat swabs of patients who came from seven districts and counties of Beijing. Sequence analysis of the 450 nucleotides of COOH-terminal of nucleoprotein （N） genes indicated that these 8 strains belonged to Hla genotype. The average genetic distances of these 8 strains to Hla genotype, Chin9322, Hlb genotype, Chin9475 and Hlc genotype, Chin9427, were 0. 004 - 0. 011, 0. 026 - 0. 031 and 0. 015 - 0.022, respectively. The average genetic distances of these 8 strains to Hla genotype, Anhui 01-1/Anhui 02-2, were 0. 000 - 0. 009 （0-5 nucleotide variation）. Conclusions Major genotypes of wild-type measles viruses circulated in Beijing in 2003 were Hla genotype. The genotypes Hlc, Hlb and 82 may have disappeared in Beijing.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2006年第5期364-368,共5页
Chinese Journal of Pediatrics
基金
北京市卫生局科研基金资助项目(353)
关键词
麻疹病毒
基因型
序列分析
Measles virus
Genotype
Sequence analysis
