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苯并噻二唑(BTH)诱导黄瓜幼苗对霜霉病抗性的研究 被引量:25

Studies on Induction Resistance to Psedoperonospora cubensis by BTH in Cucumber Seedlings
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摘要 用0.5mmol/LBTH(苯并噻二唑)溶液在接种黄瓜霜霉病菌前4、8、12、24、48、96、168h(7d)和接种后2、4d处理二叶期的黄瓜幼苗,结果表明BTH对霜霉病菌无直接的抑制作用,但能诱导黄瓜幼苗对霜霉病产生局部和系统的抗性,系统抗性的表达在处理后48h产生并持续7d以上。几丁质酶和β13葡聚糖酶活性测定结果表明,BTH处理可系统性地增强这两种酶的活性,且与黄瓜对霜霉病的诱导抗性密切相关。 0. 5 mmol/L BTH was used in seedling with two true leaves 4, 8 and 12 h, or 1, 2, 4 and 7 days before or 4 and 2 days after inoculation with P. cubensis. Results obtained showed that BTH had not a curative effect but could induce local and systemic resistance in cucumber against the downy mildew. Expression of systemic resistance in leaves was detectable 48 h after treatment and continued for at least 7 days. Chitinase and β-1, 3-glucanase activities analysis showed that induced resistance in cucumber leaves was accompanied by a systemic increasing in both enzymes activities. Two enzymes were related to resistance to downy mildew in cucumber.
出处 《园艺学报》 CAS CSCD 北大核心 2006年第2期278-282,共5页 Acta Horticulturae Sinica
基金 国家‘十五’科技攻关重大专项课题(2004BA516A09) 西北农林科技大学青年学术骨干支持计划项目 西北农林科技大学科研专项基金资助项目(042M083)
关键词 黄瓜 霜霉病 BTH 诱导抗性 几丁质酶 Β-1 3-葡聚糖酶 Cucumber downy mildew BTH Induced resistance Chitinase β-1, 3-glucanase
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